重组人促红细胞生成素对急性脑损伤大鼠的神经保护作用研究

目的：探讨重组人促红细胞生成素（rh‐EPO）对大鼠急性脑损伤后脑细胞凋亡的保护作用及对GLT‐1／GLAST表达的影响。方法60只大鼠分为对照组（n＝18）、急性脑损伤组（n＝22）和rh‐EPO处理组（n＝20），急性脑损伤组用改良Feeney′s自由落体打击器制作急性脑损伤模型，rh‐EPO处理组采用同样的方法制作脑外伤模型15 min后腹腔注射rh‐EPO ，所有动物在实验完成48 h后断头取脑。应用RT‐PCR、Western blot检测GLT‐1、GLAST mRNA及蛋白表达。结果急性脑损伤48 h后，大鼠缺血区GLT‐1和GLAST mRNA及蛋白表达较对照组均显著降低（均 P＜0．01），而rh‐EPO处理组GLT‐1和GLAST 的mRNA及蛋白表达较急性脑损伤组均显著增加（均 P＜0．01）。结论 EPO可显著减轻大鼠急性脑损伤，其机制可能与GLT‐1／GLAST表达上调有关。

The protection study of recombinant human erythropoietin on acute cerebral injury rats

Objective To investigate the effect of recombinant human erythropoietin(rh‐EPO ) on acute cerebral injury and expression of GLT‐1 and GLAST in rat .Methods Sixty SD rats were randomly divided into three groups by weight :control group (n=18) ,acute cerebral injury group(n=22) and rh‐EPO conditioning group(n=20) .Acute cerebral injury models were made by modified Feeney′s method .rh‐EPO was injected in abdominal cavity 15 min after acute cerebral injury in rh‐EPO conditioning group .Rats′brain were removed 48 h after experiments .Rat GLT‐1 and GLAST mRNA expression were determined by RT‐PCR , GLT‐1 and GLAST protein expression were determined by Western blot .Results GLT‐1/GLAST mRNA and protein expression decreased significantly after acute cerebral injury(all P<0 .01) ,but increased significantly in rh‐EPO preconditioning group com‐pared with acute cerebral injury group(all P<0 .01) .Conclusion rh‐EPO preconditioning may protect against acute cerebral injury by up regulating the expression of GLT‐1/GLAST .