1型与4型戊型肝炎病毒ORF2抗原性比较

目的 比较1型和4型HEV ORF2抗原性的差异。方法 对1型和4型ORF2同样位置各3个抗原片段分别进行表达，1型ORF2的3个重叠抗原以从N端到C端的顺序编号为1-pET-1、1-pET-2和1-pgF-3；同样4型3个片段的编号为4-pET-1、4-pET-2和4-pET-3。用纯化后的各抗原片段分别建立酶联免疫检测方法检测临床肝炎病人血清136份，对检测结果进行统计分析。结果 对6个抗原片段成功地进行了表达，纯化后6个抗原片段的纯度在95％以上。通过检测136份临床肝炎病人血清，显示1型抗原中3个抗原片段均存在抗原性差异，4型病毒ORF2中间的抗原片段与C端和N端抗原片段存在抗原性差异，1型和4型病毒之间N端抗原存在抗原性差异，具有统计学意义。结论 1型和4型HEV ORF2 N端重组蛋白之间存在抗原性差异。

Comparison of ORF2 antigenicity between HEV genotypes 1 and 4

Objective To compare ORF2 antigenicity between HEV genotypes 1 and 4. Methods Three overlapping fragments at corresponding position on ORF2 of genotypes 1 and 4 which cover the full-length of ORF2 were cloned and expressed in E.coli system. The expressed polypeptides were purified. The ELISA for each fragment was developed with the corresponding purified polypeptide. One hundred and thirty six serum samples from patients was tested. The detecting results with different ELISA method were compared and analyzed. Results The six fragments were successfully expressed in E.coli system. The purity of the six purified polypeptides was more than 95%. The results showed that three polypeptides from genotype 1 ORF2 were significantly different, the middle polypeptide from genotype 4 ORF2 was significantly different from fragments of N and C terminus, the fragments at N terminus from genotypes 1 and 4 were significantly different. Conclusion Antigenicity difference exists at N terminus of genotypes 1 and 4 ORF2.