ABCB1 gene polymorphisms and haplotype analysis in colorectal cancer |
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Authors: | Mariusz Panczyk Ewa Balcerczak Sylwester Piaskowski Krzysztof Jamroziak Gra?yna Pasz-Walczak Marek Mirowski |
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Institution: | (1) Department of Pharmaceutical Biochemistry, Laboratory of Molecular Biology and Pharmacogenomics, Medical University of Lodz, Muszynskiego 1 Street, 90-151 Lodz, Poland;(2) Department of Molecular Pathology and Neuropathology, Medical University of Lodz, Lodz, Poland;(3) Department of Haematology, Medical University of Lodz, Lodz, Poland;(4) Department of Pathology, Medical University of Lodz, Lodz, Poland |
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Abstract: | Purpose The aim of this study was to determine the significance of three most common single-nucleotide polymorphisms (SNPs) of ABCB1 gene in the development of colorectal cancer and to estimate the influence of these SNPs to surviving patients' treatment
combination adjuvant therapy 5-fluorouracil/leucovorin. Haplotype structure of ABCB1 was analysed, and degree of linkage disequilibrium (LD) between SNPs of ABCB1 was estimated.
Materials and methods Tumour specimens of 95 patients with colorectal cancer and blood samples of 95 healthy cases were studied. Genotyping of ABCB1 gene was performed by automated sequencing or polymerase chain reaction-restriction fragment length polymorphism method.
Comparison of frequencies of alleles/genotypes/haplotypes between the studied group (colorectal cancer samples) and the control
group (blood samples) were analysed. These results were correlated with the surviving patients after treatment of adjuvant
chemotherapy.
Results Significant differences in ABCB1
1236C>T (p = 0.00043) and ABCB1
2677G>T/A (p = 0.04) genotype distribution and T1236 allele distribution (CT1236 or TT1236 vs CC1236; p = 0.0499, OR = 0.55, Fi–Yule coefficient = 0.14) were found. A strong LD between ABCB1
1236C>T and ABCB1
2677G>T/A SNPs (D′ = 0.621, r
2 = 0.318) was detected. All SNPs were located in one haplotype block. There were significant differences in haplotype distributions
between colorectal cancer patients and healthy population (p = 0.03). Significant differences in survival probability of colorectal cancer patients' treatment chemotherapy according
to allele of ABCB1
3435C>T was observed. Survival probability of patients with wild-type C3435 allele were higher than among patients without this allele (p = 0.04572).
Conclusions These results suggested that three studied SNPs of ABCB1 were located in one haplotype block. Differences in ABCB1
1236C>T and ABCB1
2677G>T/A genotypes and T1236 allele distribution between investigated populations indicate significant impact of these SNPs on risk of development of
colorectal cancer. Polymorphism ABCB1
3435C>T may be a prediction marker of cancer chemotherapy effectiveness. Differences in haplotype distributions between colorectal
cancer patients and healthy population suggested that other potential SNPs, especially in regulatory region of ABCB1 gene, may influence P-glycoprotein expression and function. |
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Keywords: | ABCB1 gene MDR1 gene Polymorphism Haplotype analysis Colorectal cancer |
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