A study by the agar diffusion technique of precipitating antibody directed against blue tongue virus and its relation to homotypic neutralizing antibody |
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| Authors: | George W Klontz Sven -Eric Svehag John R Gorham |
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| Institution: | (1) Washington Agricultural Experiment Station, Washington State University, Pullman, Washington;(2) Animal Disease and Parasite Division, Agricultural Research Service, U. S. Department of Agriculture, Pullman, Washington |
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| Abstract: | Summary The technique of agar diffusion was applied to a study of blue tongue virus antigens prepared from infected mouse brains, chicken embryos, and cell culture fluid. Antigens from these different sources, contributing to precipitate formation, appeared to be virus-specific, noninfectious and serologically indistinguishable one from another in the systems tested.The onset and production of circulating ovine precipitating antibody was correlated to corresponding data for homotypic virus infectivity neutralizing antibody. The onset of circulating precipitating antibody formation was detected about the same time as for neutralizing antibody but the precipitating antibody persisted longer. An anamnestic response was observed for neutralizing antibody but not for the precipitating antibody.An antigen-antibody system containing one reagent in low concentration precipitated if adjacent to a precipitating control system but not if placed by itself or an angle >90° to the control system. This phenomenon, termed the  recruiting effect , was found to influence quantitation of precipitating antibody. |
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