小鼠L6565白血病克隆细胞株的建立及其生物学特性

目的建立Ｌ６５６５白血病克隆细胞株，为白血病的生物学、病毒学和实验治疗学提供体外研究模型。方法有限稀释法建成克隆细胞，从生长情况、染色体、形态、超微结构、Ｘ－Ｃ检测、癌基因表达及分化标志等方面了解其生物学特性。结果Ｌ６５６５细胞生长旺盛，形态大小不一，染色体条数从３８到１４４条不等，众数为４２条，有标记染色体Ａ、Ｂ、Ｍ。超微结构细胞内核仁清楚，细胞器丰富，核占３／４左右，细胞内有Ａ型和Ｃ型病毒颗粒，Ｘ－Ｃ检测阳性，ｃｍｙｃ、ｃｆｏｓ癌基因高表达，分化标志ＣＤ４和ＣＤ８阴性。结论我们成功地建立了含有致白血病病毒的Ｌ６５６５克隆细胞株，该细胞性质属于淋巴干细胞性白血病细胞。

Establishment and Biological Characteristics of Murine L6565 Leukemia Cell Clone

Purpose To establish L6565 cell clone to provide a model for the study of the biology,virology and exprerimental therapy of leukemia. Methods L6565 cell clone was established by limited dilution.To study its biological features from the growth,morphology,ultrastructure,chromosome,XC assay and expression of oncogene and differentiation was observed. Results L6565 clone cell which differnt shaps is proliferated quickly,the chromosome counts were varied from 38 to 144,and the stem line cells were 42(45 %)A,B,M marker chromoso mes were found,the electron microscopy observation demonstrates that L6565 clone cell nucleolus was clear,rich in oragnalles and virus particle type A and type C were distributed in cytoplasm;XC assay showed positive;c myc and c fos were overexpression. Conclusions We establish L6565 cell clone secucesfully,which is a stem lymphocytic leukemia cells containing RNA virus.