MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory |
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| Authors: | Etienne Carbonnelle,Cé cile Mesquita,Emmanuelle Bille,Nesrine Day,Brunhilde Dauphin,Jean-Luc Beretti,Agnè s Ferroni,Laurent Gutmann,Xavier Nassif |
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| Affiliation: | aHôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France;bHôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France;cUniversité René Descartes Paris 5, Faculté de Médecine, Paris, France;dAndromas SAS, 156 rue de Vaugirard, Paris, France |
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| Abstract: | Since the early 1980s, mass spectrometry has emerged as a particularly powerful tool for analysis and characterization of proteins in research. Recently, bacteriologists have focused their attention on the use of mass spectrometry (MS) for bacterial identification, especially Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF). Moreover, recent publications have evaluated MALDI-TOF in microbiology laboratory for routine use. MALDI-TOF-MS is a rapid, precise, and cost-effective method for identification of intact bacteria, compared to conventional phenotypic techniques or molecular biology. Furthermore, it allows identification of bacteria directly from clinical samples (blood cultures for example).The goal of this review was to update recent data concerning routine identification of microorganisms by MALDI-TOF in the clinical microbiology laboratory. |
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| Keywords: | Bacterial identification MALDI-TOF-MS Routine identification Clinical samples Spectral patterns |
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