| 白藜芦醇对TNF-α诱导类风湿关节炎成纤维样滑膜细胞的影响 |
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| 引用本文： | 田静,高洁生,陈进伟,李芬,谢希,杜金烽,王静,毛妮.白藜芦醇对TNF-α诱导类风湿关节炎成纤维样滑膜细胞的影响[J].中国中药杂志,2010,35(14):1878-1882. |
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| 作者姓名： | 田静 高洁生 陈进伟 李芬 谢希 杜金烽 王静 毛妮 |
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| 作者单位： | 中南大学湘雅二医院,湖南,长沙,410011 |
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| 基金项目： | 2009湖南中医药科研计划项目(2009065) |
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| 摘 要： | 目的:观察白藜芦醇(Res)对TNF-α诱导类风湿关节炎(RA)成纤维样滑膜细胞(FLS)增殖及凋亡的影响,探讨其对PI3K/Akt/BAD信号通路作用。方法:MTT比色法测定Res对TNF-α诱导RA FLS生长的抑制作用。流式细胞仪检测Res对RA FLS细胞周期及凋亡率的影响。Western-blot方法分析Res对TNF-α诱导RA FLS中磷酸化Akt蛋白及其下游效应分子磷酸化BAD蛋白表达的影响。结果:Res对TNF-α诱导RA FLS增殖具有抑制作用,呈时间剂量依赖性。随着Res浓度增加,RA FLS凋亡率增加,G0/G1期细胞增多,G2/M期及S期细胞减少,与TNF-α组比较,差异具有统计学意义(P0.05)。Res可抑制TNF-α诱导RA FLS磷酸化Akt蛋白以及磷酸化BAD蛋白的表达。结论:Res通过阻滞RA FLS细胞周期、下调PI3K/Akt信号通路Akt磷酸化水平发挥抑制增殖、诱导凋亡作用,Res具有治疗RA的潜在价值。
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| 关 键 词： | 白藜芦醇 滑膜细胞 增殖 凋亡 |
| 收稿时间： | 1/4/2010 12:00:00 AM |
Effects of resveratrol on proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes |
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| TIAN Jing,GAO Jiesheng,CHEN Jinwei,LI Fen,XIE Xi,DU Jinfeng,WANG Jing and MAO Ni.Effects of resveratrol on proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes[J].China Journal of Chinese Materia Medica,2010,35(14):1878-1882. |
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| Authors: | TIAN Jing GAO Jiesheng CHEN Jinwei LI Fen XIE Xi DU Jinfeng WANG Jing and MAO Ni |
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| Institution: | Xiangya Second Hospital, Central South University, Changsha 410011, China;Xiangya Second Hospital, Central South University, Changsha 410011, China;Xiangya Second Hospital, Central South University, Changsha 410011, China;Xiangya Second Hospital, Central South University, Changsha 410011, China;Xiangya Second Hospital, Central South University, Changsha 410011, China;Xiangya Second Hospital, Central South University, Changsha 410011, China;Xiangya Second Hospital, Central South University, Changsha 410011, China;Xiangya Second Hospital, Central South University, Changsha 410011, China |
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| Abstract: | |
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| Keywords: | resveratrol fibroblast-like synoviocytes proliferation apoptosis |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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