肝癌致梗阻性黄疸时丹参对ICAM-1表达的影响

目的:建立恶性梗阻性黄疸模型,通过对模型鼠腹腔内注射丹参注射液,观察其对肝癌瘤体大小、抑癌率及抑转移率和肝癌、癌周、临近肝叶及肺组织中细胞间黏附分子(ICAM-1)表达的作用.方法:用Walker-256肝癌株近肝门部肝实质内种植致移植性肝癌侵袭高位胆管,造成胆道癌性狭窄,以建立SD大鼠恶性梗阻性黄疸模型.将模型鼠分成四组,通过对模型鼠腹腔内分别注射等量的生理盐水(n=24)、肌苷 维生素C(n=40)、丹参(n=40)和5FU(n=40).观察肝癌瘤体大小、抑癌率及抑转移率和肝癌、癌周、临近肝叶及肺组织中ICAM-1的表达,并对其结果进行统计学分析.结果:丹参组与生理盐水组和肌苷 维生素C组相比,肝癌平均瘤体减小、抑癌率及抑转移率增高(P<0.01);与5FU组相比,平均瘤体却较大(P<0.01).在抑癌率及抑肝转移率方面,丹参组除抑肺转移率外与5-FU组相比无显著性差异(P＞0.05).丹参组ICAM-1的表达明显低于生理盐水组(P<0.01)、肌苷 维生素C组(P<0.01)和5FU组(P<0.05).结论:在肝癌致梗阻性黄疸时,丹参通过促进肝癌细胞成熟分化、抑制肝癌细胞增殖,降低肝癌、癌周、临近肝叶及肺组织中ICAM-1的表达,而对肿瘤发展起抑制作用.

Effects of Danshen injection on ICAM-1 expression in hepatocellular carcinoma with malignant biliary obstruction

AIM: To establish a malignant biliary obstruction model of SD rats and to study the effects of Danshen injection on the diameter of tumor, inhibitory rates and metastasic rates of hepatocellular carcinoma and on the expression of intercellular adhesion molecule-1 (ICAM-1) in carcinoma and peri-carcinoma tissues, adjacent lobes (left-internal lobe) as well as lung tissues. METHODS: Malignant biliary obstruction model of SD rats was made by vaccination of transplanted tumor in liver near the porta hepatis with walker-256 hepatocarcinoma cell line infiltrating and metastasizing to proximal common bile duct. The SD rats were divided into four groups and each group were treated respectively by 0.9% NS (n=24), by inosine+Vitamin C (n=40), by Danshen (n=40) and by 5-FU (n=40). Observations were made on the diameter of the tumors, the inhibitory rates, the metastasic rates of hepatocellular carcinoma and the expression of ICAM-1 in carcinoma and peri-carcinoma tissues, adjacent lobes (left-internal lobe) as well as lung tissues. Statistical analysis was conducted. RESULTS: The diameters of tumors were reduced and both the tumor and metastasis inhibitory rates of hepatocellular carcinoma were enhanced after the treatment of Danshen injection, compared with those in rats treated by 0.9% NS and inosine combined with Vitamin C (P<(0.01)). The inhibitory rates(41.7%) and liver's and lung's metastasic rates (59.7% and 81.1%) of hepatocellular carcinoma in rats treated by Danshen injection were similar to those in rats treated by 5-FU (P>0.05) except lung's metastasic rates (P<0.01). The expression of ICAM-1 in carcinoma, peri-carcinoma adjacent lobe tissues as well as lung tissues was significantly reduced after treatment of Danshen injection compared with that in rats treated by (0.9%) NS and inosine combined with Vitamine C (P<0.01) and in rats treated by 5-FU (P<0.05). CONCLUSION: In SD rat model of malignant biliary obstruction, Danshen, can inhibit the growth of hepatocarcinoma through reducing proliferation of hepatocarcinoma cells, increasing the reversion of hepatocarcinoma cells and decreasing the expression of ICAM-1 in carcinoma, peri-carcinoma adjacent lobe tissues as well as lung tissues.