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| 高效液相色谱法测定人血清中马兜铃酸Ⅰ、Ⅱ的含量 | |
| 引用本文： | 朱光辉,王奇志,王增寿,许菲菲. 高效液相色谱法测定人血清中马兜铃酸Ⅰ、Ⅱ的含量[J]. 中国药房, 2006, 17(18): 1397-1398 |
| 作者姓名： | 朱光辉 王奇志 王增寿 许菲菲 |
| 作者单位： | 1. 温州医学院附属二院药剂科,温州市,325027 2. 温州医学院药学院,温州市,325035 3. 温州医学院附属一院肾内科,温州市,325000 |
| 摘 要： | 目的建立以高效液相色谱法测定人血清中马兜铃酸Ⅰ、Ⅱ含量的方法。方法色谱柱为C18(250mm×4.6mm,5μm),流动相为甲醇-水-冰醋酸(72∶27∶1),检测波长为250nm,流速为1.0ml/min。结果马兜铃酸Ⅰ、Ⅱ检测浓度线性范围分别为0.84~13.50μg/ml(r=0.9997,n=5)、2.03~32.50μg/ml(r=0.9994,n=5),最低检测浓度分别为0.3、0.1μg/ml。结论本方法简便、易行,可为临床诊治马兜铃酸肾病提供依据。 |
| 关 键 词： | 高效液相色谱法 马兜铃酸Ⅰ 马兜铃酸Ⅱ 血药浓度 |
| 文章编号： | 1001-0408(2006)18-1397-02 |
| 修稿时间： | 2006-05-15 |
Determination of Aristolochic Acid Ⅰ and Aristolochic Acid Ⅱ in Human Serum by HPLC |
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| ZHU Guanghui,WANG Qizhi,WANG Zengshou,XU Feifei. Determination of Aristolochic Acid Ⅰ and Aristolochic Acid Ⅱ in Human Serum by HPLC[J]. China Pharmacy, 2006, 17(18): 1397-1398 | |
| Authors: | ZHU Guanghui WANG Qizhi WANG Zengshou XU Feifei |
| Abstract: | OBJECTIVE:To establish a HPLC method for the determination of aristolochic acidⅠand aristolochic acid Ⅱin human serum.METHODS:The separation was performed on C 18 column(250mm×4.6mm,5μm).The mobile phase consisted of methanol-water-acetic(72∶27∶1)at a flow rate of1.0ml/min.The detection wavelength was250nm.RESULTS:The linear ranges for aristolochic acidⅠand aristolochic acid Ⅱ were0.84～13.50μg/ml(r=0.9997,n=5)and2.03～32.50μg/ml(r=0.9994,n=5),respectively.The lowest determination concentration of aristolochic acid Ⅰ and aristolochic acid Ⅱ were0.3μg/ml and0.1μg/ml,respectively.CONCLUSION:The method is simple and practicable,and it can provide basis for clinical diagnosis and treatment of aristolochic acid nephropathy. |
| Keywords: | HPLC Aristolochic acid Ⅰ Aristolochic acid Ⅱ Serum concentration |
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