HPCE-DAD同时测定罗布麻叶中4种黄酮的含量

 目的 建立高效毛细管电泳二极管阵列检测法(HPCE-DAD) 同时测定不同产地、采收期和种属的罗布麻叶中芦丁、异槲皮苷、金丝桃苷及槲皮素含量的方法。方法 选择毛细管区带电泳分离模式，以45 mmol·L-1硼砂缓冲液(pH 9.2)为电泳介质，未涂渍标准熔融石英毛细管(64.5 cm×75 μm， 有效长度56 cm)为分离通道，分离电压为25 kV，检测波长为206 nm，毛细管温度为25 ℃，压力进样为5 000 Pa，5 s。结果 4种黄酮的峰面积与浓度的线性关系良好(r>0.998 6) ；加样回收率为95.34%～102.65%。结论 该方法简单、准确，重现性较好， 可用于罗布麻叶药材内在质量的评价和控制。

Simultaneous Determination of Four Flavonoids in Folium Apocyni Veneti by HPCE-DAD

OBJECTIVE To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) for simultaneous determination of rutin， isoquercitrin， hyperin and quercetin in folium apocyni veneti from different habitats，harvesting time and species. METHODS Based on the mode of capillary zone electrophoresis，45 mmol·L-1 borax was used as buffer solution (pH 9.2) ， uncoated fused silica capillary (64.5 cm×75 μm id) was used， separation voltage was 25 kV ， detection wavelength was at 206 nm， column temperature was maintained at 25 ℃，and sample was injected at 5 000 Pa for 5 s.RESULTS Four flavonoids showed good linearity (r>0.998 6) in the range of the tested concentration. The average recoveries of the method were in the rang of 95.34%-102.65%.CONCLUSION The method is simple， accurate and reproducible， and can be used for quality control of Folium Apocyni Veneti.