大蒜治疗口腔癌前病变的机理探讨

目的:探讨大蒜治疗口腔癌前病变及其机理。方法:用0.5%4-硝基喹啉氧化物涂于 Wistar 大鼠硬腭后方,每周3次,7周后建立口腔粘膜癌前病变模型。然后将大鼠随机分为治疗组和对照组。于治疗组大鼠硬腭后方涂大蒜注射液,对照组涂等量蒸馏水,每周3次,共8周。分别于第5、8周及停药后第7周分批处死动物。不同时期取腭粘膜标本进行光镜及电镜观察。结果:治疗组无1例恶化,而对照组3个时期的恶化率分别为25.0%、16.7%、14.3%。超微结构研究表明大蒜能抑制细胞的活跃增生。结论:大蒜能阻断口腔癌前病变的发展,抑制细胞活跃增生(P<0.01),但不能使癌前病变逆转为正常组织(P>0.05)。

Experimental Study of Garlic in Treatment on Oral Precancer

Objective:To determine the effects of garlic on oral precancer.Methods:The posterior hard palatal mucosae of 42 Wistar rats were treated three times weekly with 0.5% 4-Nitroquinolin-1-oxide in dimethyl sulfoxide for 7 weeks by coat- ing method.Then the biopsy was taken and indicated that 58.3% animals suffered slight degree of epithelial dysplasia. After that the animals were divided randomly into two groups.The treatment group(n=21)was treated three times weekly with garlic injection,at the posterior hard palatal mucousae by coating method,and in the control group(n=21), the vehicle-distilled water was instead of garlic injection.The treatment period was 8 weeks.The animals were killed at the 5th,8th week,and 7 weeks after treatment respectively.The palatal sample was examined with light-microscopy (LM)and transmission electron microscopy(TEM).Results:No deterioration cases were found in the treatment group, while in the control group,5th,8th and 7th week after the treatment,the deterioration eases were 2.5%,16.7% and 14.3% respectively.Ultrastructureal study showed that garlic inhibits cell hyperplasia and blocks the epitheliar dysplasia (P<0.01).Conclnsion:Garlic could block posterior hard palatal mucoeal epithelial dysplasia,but couldn't make the ep- ithelial dysplasia reverse to normal.