口腔粘膜癌前病变纽胞核DNA含量的定量分析

采用流式细胞光度术(FCM)对51例口腔粘膜样本进行了细胞核DNA含量及细胞周期分析,发现由正常组织、单纯增生、轻增、重增到鳞癌的DNA含量及S时相细胞的百分比是逐渐增加的。异常增生与正常组织、单纯增生相比差异有显著性。异常增生和鳞癌中有异倍体出现,轻增、重增、鳞癌的异倍体出现率分别为40%,50%70%。结果表明,口腔粘膜由正常组织、单纯增生、轻增、重增到鳞癌是一个连续的发展过程,其增殖活性逐渐升高。DNA含量和S时相百分比是反映异常增生和癌变的客观指标。

DNA CONTENTS IN PREMALIGNANT LESIONS OF ORAL MUCOSA:A QUANTITATIVE STUDY

Fifty-one samples of oral mucosa were detected by FlowCytometry(FCM)to evaluate the DNA contents of nucleus and analyse thecell cycle.The results showed that the DNA contents and percentage ol Sphase cells increased gradually from normal mucosa,simple hyperplasia,milddysplasia,severe dysplasia to squamous cell carcinoma.The two indexes indysplasia were both higher significantly than that in normal mucosa orhyperplasia.The percentage of S phase cell in mild dysplasia was lower signi-ficantly than that in squamous cell carcinoma.Aneuploid could be found indysplasia and squamous cell carcinoma,the aneuploid rate of mild dysplasia,Sever dysplasia,squamous cell carcinoma was 40%,50%,70%,respectively.The results suggested the DNA contents and the percentage of S phase cellsare relatively objective indexes for the evaluate of dysplasia and squamouscell carcinoma.FCM can improve the accuracy of diagnosis in the premalig-nant lesions of oral mucosa.