大孔树脂分离纯化半枝莲总黄酮的研究

目的研究不同型号大孔树脂纯化半枝莲总黄酮的工艺条件及参数。方法以静态饱和吸附量、洗脱量、静态洗脱率为考察指标,比较了5种大孔树脂,并对AB-8树脂的动态比上柱量、比吸附量、比洗脱量进行了考察,以总黄酮转移率和纯度为指标对树脂吸附工艺条件进行了筛选。结果所比较的5种树脂中,AB-8型树脂具有最佳的吸附及洗脱参数,其最佳工艺是用浓度为3.12mg·mL^-1,pH=2.0的上样液,以3倍柱体积去离子水,12倍柱体积70%乙醇依次洗脱,总黄酮转移率为80%左右,其纯度为60%左右。结论AB-8树脂综合性能较好,适合于半枝莲中总黄酮的纯化。

Separation and purification of total flavanoids from Scutellaria barbata by macroreticular resin

Objective To investigate the technology for adsorbing and separating total flavanoids from Scutellaria barbata with different types of macroporous resin （MR）. Methods The static capacity adsorption, static elution ratio of 5 types of MR were studied respectively and compared to find the optimum one. The dynamic saturation ratio and dynamic absorption ratio of AB-8 and the yield, and purification degree of total flavanoids were researched and compared to obtain the optimum technological parameters. Results The AB-8 type of MR possessed optimum adsorption and elution parameters. The concentration of Scutellaria barbata sample extract was 3.12 mg · mL^-1 and its pH was 2.0. After being eluted with 3 multiple column volume of distilled yield of total flavanoids was 80% and the product purity was comprehensive adsorption, and it could be used to purify the water, 12 multiple column volume of 70% ethanol, the 60%. Conclusion The AB-8 type of MR showed better extract of Scutellaria barbata.