| 氯氧甲苯咪唑诱导白血病细胞凋亡的实验研究 |
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| 引用本文： | 刘芳,邹萍,张敏,吴耀辉,肖娟. 氯氧甲苯咪唑诱导白血病细胞凋亡的实验研究[J]. 中国实验血液学杂志, 2005, 13(3): 391-393 |
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| 作者姓名： | 刘芳 邹萍 张敏 吴耀辉 肖娟 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院,血液病研究所,武汉,430022 |
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| 基金项目： | 国家自然科学基金资助项目(编号30370595) |
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| 摘 要： | 为了研究氯氧甲苯咪唑(econazole)诱导鼠粒单核白血病细胞WEHI-3凋亡的机制，利用Annexin-V／PI双染实验测定细胞凋亡，Fura-2荧光负荷技术检测细胞内游离钙离子浓度([Ca^2 ]i)，并抽取WEHI-3细胞内质网部分蛋白，利用Western blot测定caspase-7、caspase-12的蛋白表达。结果显示ieconazole作用后WEHI-3出现典型的凋亡改变，细胞内游离[Ca^2 ]i较对照明显升高，caspase-12和caspase-7表达随着细胞内钙离子浓度的提高而逐渐增加。结论：econazole能诱导WEHI-3细胞凋亡，caspase-12在此过程中起重要作用。
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| 关 键 词： | 氯氧甲苯咪唑 细胞凋亡 细胞质[Ca^2＋]i caspase-12 |
| 文章编号： | 1009-2137(2005)03-0391-03 |
| 修稿时间： | 2004-08-16 |
Experimental Study on Apoptosis in Leukemia Cells Induced by Econazole |
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| LIU Fang,ZOU Ping,ZHANG Ming,WU Yao-Hui,XIAO Juan. Experimental Study on Apoptosis in Leukemia Cells Induced by Econazole[J]. Journal of experimental hematology, 2005, 13(3): 391-393 |
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| Authors: | LIU Fang ZOU Ping ZHANG Ming WU Yao-Hui XIAO Juan |
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| Affiliation: | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. |
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| Abstract: | To investigate apoptosis of mouse leukemia cell (WEHI-3) induced by econazole and its mechanism, apoptosis induced by econazole was examined by flow cytometry, while free calcium ([Ca 2 ]i) was determined by Fura-2 fluorescein load techinique. The protein was isolated from endoplasmic reticulum of WEHI-3 cells, and then the expression of caspase-12 and caspase-7 was evaluated by Western blot. The results showed that WEHI-3 exhibited typical change of apoptosis when it was treated by econazole, [Ca 2 ]i was significantly higher in comparison with the control. The expression of caspase-12 and caspase-7 enhanced as the econazole concentration incrcased. In conclusion, econazole can induce WEHI-3 cell apoptosis and the caspase-12 plays a key role in this process. |
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| Keywords: | econazole apoptosis [Ca 2 ]i caspase-12 |
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