| 慢性髓性白血病和急性淋巴细胞白血病细胞中Wnt信号通路活性的差异 |
| |
| 引用本文： | 李庆昌,谢成耀,刘树立,方长青,赵忱.慢性髓性白血病和急性淋巴细胞白血病细胞中Wnt信号通路活性的差异[J].中华肿瘤防治杂志,2010,17(23). |
| |
| 作者姓名： | 李庆昌 谢成耀 刘树立 方长青 赵忱 |
| |
| 作者单位： | 中国医科大学基础医学院病理教研室中国医科大学附属第一医院病理科,辽宁,沈阳,110001 |
| |
| 摘 要： | 目的:探讨慢性髓性白血病(CML)和急性淋巴细胞白血病(ALL)细胞中Wnt信号通路活性是否存在差异.方法:利用慢病毒基因转染法在经BCR-ABL融合基因诱导形成的CML和ALL细胞中建立Wnt信号通路报告系统BAT-RED,检测2种白血病细胞中Wnt信号通路活性;PCR方法检测2种细胞中BAT-RED基因整合情况;通过向培养细胞中添加氯化锂,人为增强Wnt信号活性,反向验证Wnt信号通路活性在2种细胞中存在差异.结果:PCR结果显示2种白血病细胞都稳定整合了Wnt信号通路报告基因BAT-RED,在42%(188/448)的慢性粒细胞白血病细胞中可以检测到Wnt信号通路被激活,而ALL细胞仅散在少数细胞中可以检测到Wnt信号通路报告基因BAT-RED被激活;添加氯化锂后,ALL细胞中Wnt信号通路活性增强.结论:CML细胞中Wnt通路活性明显高于ALL细胞,提示Wnt信号通路在2种类型白血病细胞的增殖和演进过程中具有不同作用.
|
| 关 键 词： | 白血病 髓样 慢性期 白血病 淋巴细胞 急性 信号传导 |
Activity of Wnt signalling in CML and ALL cells |
| |
| LI Qing-chang,XIE Cheng-yao,LIU Shu-li,FANG Chang-qing,ZHAO Chen.Activity of Wnt signalling in CML and ALL cells[J].Chinese Journal of Cancer Prevention and Treatment,2010,17(23). |
| |
| Authors: | LI Qing-chang XIE Cheng-yao LIU Shu-li FANG Chang-qing ZHAO Chen |
| |
| Institution: | LI Qing-chang,XIE Cheng-yao,LIU Shu-li,FANG Chang-qing,ZHAO ChenDepartment of Pathology,College of Basic Medical Sciences,Department of Pathology,First Hospital of China Medical University,Shenyang 110001,P.R.China |
| |
| Abstract: | OBJECTIVE:To investigate whether Wnt signalling was differently utilized in chronic myelogenous leukemia(CML) versus acute lymphocytic leukemia(ALL) cells.METHODS: BCR-ABL fusion DNA fragment was introduced into mice bone marrow cells with intent to induce CML and ALL transformation,then Wnt reportor sysytem BAT-RED was successfully transfected into both CML and ALL cells with the lentiviral gene transduction method,and then the activity of Wnt signalling was evaluated in these two types of leukemia cells.T... |
| |
| Keywords: | Leukemia myeloid chronic-phase leukemia lymphocytic acute signal transduction |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
