| RNAi沉默CDX2基因对急性髓细胞白血病细胞增殖影响的实验研究 |
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| 引用本文： | 王东宁,黎国伟,叶玉蝶,吴红梅,许先吟,陈立,余相. RNAi沉默CDX2基因对急性髓细胞白血病细胞增殖影响的实验研究[J]. 中华肿瘤防治杂志, 2010, 17(22) |
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| 作者姓名： | 王东宁 黎国伟 叶玉蝶 吴红梅 许先吟 陈立 余相 |
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| 作者单位： | 1. 中山大学附属第三医院血液科,广东,广州,510630
2. 惠州市中心人民医院血液科,广东,惠州,516001 |
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| 基金项目： | 广东省医学科学研究基金 |
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| 摘 要： | 目的:探讨RNA干扰技术沉默CDX2基因对人急性髓细胞白血病细胞系THP1增殖影响的意义.方法:针对CDX2mRNA序列设计合成2对编码小干扰RNA(siRNA)的DNA模板,构建pGenSi1-CDX2siRNA重组质粒,转染THP1细胞.采用蛋白质印迹法检测重组质粒对CDX2蛋白表达的影响,MTT法观察THP1细胞增殖情况,Annexin-V-PI双染法流式细胞术检测转染重组质粒后细胞凋亡状况.结果:成功构建pGenSil-CDX2 siRNA重组质粒,成功转染THP1细胞,并能特异地抑制CDX2基因的表达;转染重组质粒后,THP1的活力分别降低为(39.8±5.0)%,与空质粒组组间差异有统计学意义,P=0.016.重组质粒组的THP1细胞凋亡率为37.3%～42.1%.结论:pGenSil-CDX2 siRNA重组质粒明显下调XDX2在急性髓细胞白血病细胞中的表达,并抑制肿瘤细胞生长,促进其凋亡.
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| 关 键 词： | 白血病,髓样,急性/病理学 转录因子 基因 细胞凋亡 |
Effects of RNA interference to CDX2 gene on proliferation of human AML cell |
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| WANG Dong-ning,LI Guo-wei,YIE Yu-die,WU Hong-mei,XU Xian-yin,CHEN Li,YU Xiang. Effects of RNA interference to CDX2 gene on proliferation of human AML cell[J]. Chinese Journal of Cancer Prevention and Treatment, 2010, 17(22) |
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| Authors: | WANG Dong-ning LI Guo-wei YIE Yu-die WU Hong-mei XU Xian-yin CHEN Li YU Xiang |
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| Affiliation: | WANG Dong-ning1,LI Guo-wei2,YIE Yu-die2,WU Hong-mei2,XU Xian-yin2,CHEN Li2,YU Xiang21.Deptartment of Hematology,Third Affiliated Hospital,Sun Yet-sen University,Guangzhou 510630,P.R.China2.Deptartment of Hematology,Huizhou Municipal Central Hospital,Huizhou 516001,P.R.China |
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| Abstract: | OBJECTIVE:To study the effects of RNA interference to CDX2 gene on proliferation of human AML cell.METHODS:A eukaryotic expression vector pGenSil was used to carry out siRNA.The DNA inserts were synthesized to target CDX2 gene.By cloning technique,pGenSil-CDX2 siRNA was constructed and confirmed by restriction cutting.AML cell(THP1 cell) was used for experiment.The expression of CDX2 was detected by Wester blot;MTT and FCM were used to observe the proliferation and apoptosis of THP1 cells.RESULTS:pGenSil-CD... |
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