Tau protein after delta-9-tetrahydrocannabinol in a human neuroblastoma cell line |
| |
| Affiliation: | 1. Hormone Laboratory, Department of Laboratory Medicine and Pathology, Haukeland University Hospital, Bergen, Norway;2. Norwegian Multiple Sclerosis Competence Centre, Department of Neurology, Haukeland University Hospital, Bergen, Norway;3. Norwegian Multiple Sclerosis Registry and Biobank, Department of Neurology, Haukeland University Hospital, Bergen, Norway;4. KG Jebsen MS Research Centre, Department of Clinical Medicine, University of Bergen, Bergen, Norway;5. Department of Neurology, Akershus University Hospital, Lørenskog, Norway;6. Institute of Clinical Medicine, University of Oslo, Oslo, Norway;7. Helse Sør-Øst Health Services Research Centre, Akershus University Hospital, Lørenskog, Norway;8. Multiple Sclerosis Centre Hakadal, Hakadal, Norway;9. Department of Medical Biochemistry, St. Olav''s Hospital, Trondheim University Hospital, Norway;10. Clinic of Laboratory Medicine, St. Olav''s Hospital, Trondheim University Hospital, Trondheim, Norway;11. Department of Laboratory Medicine, Children''s and Women''s Health, Norwegian University of Science and Technology, Trondheim, Norway;12. Department of Neurology, St. Olav''s Hospital, Trondheim University Hospital, Trondheim, Norway;13. Curato Oslo, Oslo, Norway;14. Department of Neurology, Molde Hospital, Norway;15. Unit for Applied Clinical Research, Norwegian University of Science and Technology, Trondheim, Norway;p. Unilabs Drammen, Drammen, Norway;q. Department of Neuroradiology, Oslo University Hospital Rikshospitalet, Oslo, Norway;r. Research Institute of Internal Medicine, Oslo University Hospital Rikshospitalet, Oslo, Norway;s. Section for Clinical Immunology and Infectious Diseases, Oslo University Hospital Rikshospitalet, Oslo, Norway;t. KG Jebsen Inflammatory Research Center, University of Oslo, Oslo, Norway;u. K.G. Jebsen Thrombosis Research and Expertise Center, University of Tromsø, Tromsø, Norway;v. Department of Clinical Science, University of Bergen, Bergen, Norway;1. College of Oceanology, Harbin Institute of Technology (Weihai), Weihai 264209, China;2. School of Marine Sciences, Ningbo University, Ningbo, 315211, China |
| |
| Abstract: | - 1.1. A human neuroblastoma cell line, SH-SY5Y, was used to determine the effects of delta-9-tetrahydrocannabinol (THC) on microtubule-associated tau protein.
- 2.2. After 48-hr treatment, THC (10−9 M) decreased 50 kD tau protein in the cytoplasmic (supernatant) fraction, and in the membrane (pellet) fraction the drug (10−7 M) also decreased 50 kD tau protein.
- 3.3. This reduction in tau protein was accompanied by a 27% reduction (P<0.05) in the membrane (pellet) total protein after (10−7 M) THC and a 28% increase (P<0.02) in cytoplasmic (supernatant) total protein after 10−9 M THC.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
