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| CXCR7-shRNA慢病毒载体对人肝癌细胞生长及侵袭能力的抑制作用 | |
| 引用本文： | 戴小珍,熊新,王兰,潘克俭,何浪,李红.CXCR7-shRNA慢病毒载体对人肝癌细胞生长及侵袭能力的抑制作用[J].南方医科大学学报,2013,33(7):994-998. |
| 作者姓名： | 戴小珍 熊新 王兰 潘克俭 何浪 李红 |
| 作者单位： | 戴小珍 (成都医学院生物医学系,四川成都610500重庆大学生物流变科学与技术教育部重点实验室,重庆400044); 熊新 (重庆医科大学第一附属医院实验研究中心,重庆,400016); 王兰 (成都医学院生物医学系,四川成都,610500); 潘克俭 (成都医学院生物医学系,四川成都,610500); 何浪 (成都医学院生物医学系,四川成都,610500); 李红 (成都医学院生物医学系,四川成都,610500); |
| 基金项目： | 国家自然科学基金(项目编号：81200917,81201702)四川省教育厅资助科研项目(项目编号：10ZC092)重庆市自然科学基金项目(项目编号：cstc2012jjA10139)重庆大学"生物流变科学与技术"教育部重点实验室访问学者基金(CQKLBST-2012-003)Supported,by,National,Natural,Science,Foundation,of,China(项目编号：81200917,81201702) |
| 摘 要： | 目的探讨CXCR7-shRNA慢病毒表达载体靶向抑制CXCR7的表达对人肝癌细胞增殖及侵袭能力的影响。方法利用 RNA干扰技术，构建CXCR7的shRNA慢病毒表达载体，转染人肺转移肝癌细胞株HCCLM3，分别通过RT-PCR和Western blot 检测HCCLM3细胞中CXCR7 mRNA和蛋白质表达水平的变化；然后通过MTT法考察CXCR7沉默对HCCLM3细胞增殖侵袭 能力的影响，通过体外粘附实验和Transwell小室法分别考察CXCR7沉默对HCCLM3细胞粘附及侵袭能力的影响。结果与空 白对照组比较，转染CXCR7-shRNA慢病毒载体的HCCLM3细胞中CXCR7 的mRNA及蛋白水平表达均明显降低（P<0.01）， SDF-1诱导的HCCLM3细胞的增殖能力显著下降（P<0.05），同时HCCLM3细胞的粘附及侵袭能力也明显受到CXCR7-shRNA 的抑制（P<0.01）。结论靶向沉默CXCR7能显著抑制结肝癌细胞的增殖和侵袭能力，为肝癌的治疗提供一个潜在的作用靶点。 |
| 关 键 词： | 肝癌 CXCR7-shRNA 生长 侵袭 |
Effects of CXCR7-shRNA lentiviral vector on the growth and invasiveness of human hepatoma carcinoma cells in vitro |
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| DAI Xiaozhen,XIONG Xin,WANG Lan,PAN Kejian,HE Lang,LI Hong.Effects of CXCR7-shRNA lentiviral vector on the growth and invasiveness of human hepatoma carcinoma cells in vitro[J].Journal of Southern Medical University,2013,33(7):994-998. | |
| Authors: | DAI Xiaozhen XIONG Xin WANG Lan PAN Kejian HE Lang LI Hong |
| Institution: | 1 School of Biomedicine, Chengdu Medical College, Chengdu 610500, China; 2 Key Laboratory of Biorheological Science and Technolo (Chongqing University), Ministry of Education, Chongqing 400044, China; 3 Laboratory Research Center, First Affiliated Hospit Chongqing Medical University, Chongqing 400016, China |
| Abstract: | Objective To explore the effects of CXCR7 knock-down by CXCR7-shRNA lentiviral vector on the proliferation and invasion of human hepatoma carcinoma cells in vitro. Methods CXCR7-shRNA lentiviral vector was transfected into heptatocellular carcinoma HCCLM3 cells. The changes in mRNA and protein expression of CXCR7 in the transfected cells were investigated using real-time PCR and Western blotting, respectively, and MTT assay was employed to assess the cell proliferation changes. In vitro adhesion assay and transwell chamber test were used to observe the adhesion and invasiveness of HCCLM3 cells, respectively. Results Transfection of HCCLM3 cells with CXCR7-shRNA lentiviral vector resulted in a significantly decreased expression of CXCR7 at both mRNA and protein levels (P<0.01) and obvious suppression of the cell proliferative activity (P<0.05). CXCR7-shRNA also significantly suppressed the invasiveness and adhesion of HCCLM3 cells (P< 0.01). Conclusion CXCR7 knock-down can significantly inhibit the proliferation and invasiveness of human hepatoma carcinoma cells in vitro, suggesting the value of CXCR7 as a potential target for hepatoma carcinoma therapy. |
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