Inconsistent association of Epstein-Barr virus with CD56 (NCAM)-positive angiocentric lymphoma occuring in sites other than the upper and lower respiratory tract |
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Authors: | Y KOBASHI S NAKAMURA Y SASAJIMA T KOSHIKAWA Y YATABE K KITOH S MORI R UEDA H YAMABE & T SUCHI |
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Institution: | Department of Pathology, Tenri Hospital, Nara,;Department of Pathology and Clinical Laboratories, Aichi Cancer Center Hospital, Nagoya,;Department of Pathology, Institute of Medical Science, University of Tokyo,;Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Nagoya,;Laboratory of Anatomic Pathology, Kyoto University Hospital, Kyoto, Japan |
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Abstract: | We previously described nine cases of angiocentric lymphoma of a possible natural killer (NK)-cell lineage with a surface CD3− CD56+ phenotype occurring in sites other than the upper and lower respiratory tract. This study was performed to investigate the association of Epstein-Barr virus (EBV) with these lymphomas, using the polymerase chain reaction (PCR) for the presence of EBV-DNA, in situ hybridization (ISH) for EBV-encoded small RNAs (EBERs) and immunohistology for EBV-determined nuclear antigen-2 (EBNA-2) and latent membrane protein-1 (LMP-1) in paraffin sections. PCR and ISH produced almost identical results, and EBERs were identified in the nuclei of the lymphoma cells of three cases, two of which exhibited LMP-1 in the cytoplasm of tumour cells without EBNA-2 expression. Molecular genetic analysis revealed EBV to be incorporated into these three EBER-positive cases either clonally or biclonally. It was revealed by re-evaluation of their morphology with the established EBV status on each case that, in contrast to the rather variable and irregular cellular composition of the EBV- positive tumours, the EBV-negative tumours stood out because of their remarkably uniform 'blastoid' appearance, and could be grouped as blastic NK-cell lymphoma. The relationship of the EBV-positive cases with nasal NK-cell tumours has yet to be clarified. |
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Keywords: | CD56 (NCAM) angiocentric lymphoma natural killer cell Epstein-Barr virus blastic NK-cell lymphoma |
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