肝郁脾虚证肝纤维化大鼠模型研究

目的建立肝郁脾虚证肝纤维化动物模型，为中医药辨证论治肝纤维化提供研究平台。方法采用四氯化碳皮下注射附加夹尾刺激、饥饱失常等制备肝郁脾虚证肝纤维化大鼠模型，根据症状表现记分判断证候的符合率，检测全血粘度、红细胞聚集指数、纤维蛋白原含量等指标确定瘀血程度；肝组织病理学检查判断肝损害状况和纤维化程度；用疏肝健脾活血方加味四逆散进行反证性治疗。结果肝郁脾虚证大鼠证候表现的符合率达82．5％；各组大鼠的全血粘度、红细胞聚集指数、纤维蛋白原含量都有不同程度的升高；治疗组大鼠证候表现、瘀血指标、肝组织损害及纤维化程度有显著改善。结论采用四氯化碳注射附加夹尾刺激、饥饱失常等中医因素可制备出符合率高而典型的肝郁脾虚证肝纤维化动物模型；加味四逆散反证性治疗有；明显疗效，提示证候模型的成功。

Study of Animal Model of Hepatic Fibrosis with Liver Stagnation and Spleen Deficiency Syndrome in Rats

Aim: To establish animal model of hepatic fibrosis with Liver stagnation and spleen deficiency syndrome in rats to provide research condition of treating hepatic fibrosis with TCM treatment based on syndrome differentiation.Method: Methods like subcutaneous injection of CCl4,clipping tail stimulation and improper diet causing hunger and fullness were used to establish rat model of hepatic fibrosis with Liver stagnation and spleen deficiency syndrome.And then the patterns were judged based on symptom manifestation score.The blood stasis degree was judged according to indexes such as whole blood viscosity,aggregation index of RBC and fibrinogen concentration of plasma.The condition of liver impairment and fibrosis degree were determined by pathological examination.Jia wei si ni san with function of soothing liver and invigorating blood was used as counterevidence treatment.Result: Coincidence rate of Liver stagnation and spleen deficiency syndrome model was 82.5%.The whole blood viscosity,aggregation index of RBC and fibrinogen concentration of plasma in all groups of rats were raised with different degree.The syndrome manifestation,blood stasis index,condition of liver impairment and fibrosis degree of treatment group rats were significantly improve.Conclusion: subcutaneous injection of CCl4,clipping tail stimulation and improper diet causing hunger and fullness established high coincidence rate and typical animal model of hepatic fibrosis with Liver stagnation and spleen deficiency syndrome in rats.Counterevidence treatment of Jia wei si ni san had obvious effect which showed the successful establishment of animal model.