犬化学去细胞神经同种异体移植的神经再生研究

目的 观察犬去细胞异体神经移植后近期神经再生的情况。谅15只家犬分成实验组（去细胞神经移植组，6只犬），对照组I（自体神经移植组，6只犬），对照组Ⅱ（新鲜异体神经移植组，3只犬），制成犬坐骨神经缺损5.0cm的模型，以上述3种神经移植物桥接修复。术后6个月行神经再生的组织学观察。结果 实验组移植段内有大量的新生神经纤维，新生血管及雪旺细胞；远端胫神经内出现大量的有髓神经纤维；靶肌肉运动终板的数量，形态及分布均良好。实验组和对照组I非常相似。结论 化学去细胞神经作为同种异体神经移植物，在修复术的粗大和长段神经缺损时不会被宿主排斥和吸收，其神经再生与自体神经移植无明显差别。

Study of nerve regeneration after transfer of acellular allografting nerve by chemical extraction at canines

Objective To observe the short term nerve regeneration in the canines after allografting of nerve made acellular through chemical extraction.Methods 15 canines were divided into experimental group (6 cases), control group I(autografting group , 6 cases ) and control group II (fresh nerve allografting group , 3 cases). The model of defects of sciatic nerves (5.0 cm in length) was set up. The defect was bridged by the above 3 nerve grafts respectively. The histological observation for the nerve regeneration was done at postoperative 6 month.Results There was a mass of regenerating nerve, vessel and Schwann cells in the grafting nerve. A multitude of myelinated nerve fiber occurred at the tibial nerve at the distal part. The number, structure and distribution of the end plate of targeted muscle were close to normal. Conclusions Chemical acellular nerve allografting would not be repulsed and absorpted by the recipient nerve when repair of large caliber and long nerve defects was done. There was no marked difference of nerve regeneration when compared with transfer of autografting nerve.