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间接凝集反应的模拟方法
引用本文:苏盛通,陈惠业.间接凝集反应的模拟方法[J].免疫学杂志,2004,20(Z1):167-169.
作者姓名:苏盛通  陈惠业
作者单位:广西玉林市卫生学校检验教研室,广西,玉林,537000
基金项目:广西教育科学“十五”规划课题资助项目
摘    要:目的节约实验经费并解决阳性标本来源困难的问题。方法用聚醋酸乙烯乳液与CaCl2溶液做乳凝试验;用人红细胞与鞣酸溶液或植物血凝素(PHA)做血凝试验;用活性炭或纸灰与鸡蛋清或氯化钙-鞣酸溶液做炭凝试验。结果乳凝试验、血凝试验、炭凝试验均得到满意的凝集效果。结论采用本法可做为乳凝、血凝、炭凝三种间接凝集反应的模拟方法,经济有效地开出有关的免疫学教学试验。

关 键 词:胶乳凝集  红细胞凝集  炭粒凝集  模拟
文章编号:1000-8861(2004)03(S)-0167-03
修稿时间:2003年8月22日

Methods of simulating the indirect agglutination
SU Sheng-tong,CHEN Hui-ye.Methods of simulating the indirect agglutination[J].Immunological Journal,2004,20(Z1):167-169.
Authors:SU Sheng-tong  CHEN Hui-ye
Abstract:Objective To economize experiment costs and to solve the problem that the positive sample source is difficult to obtain. Methods Latex agglutination was carried out using gather acetic acid ethylene latex and CaCl2 solution; hemagglutination test was carried out in the presence of human erythrocyte and tannic acid solution/phytohemagglutinin (PHA); carbon particle agglutination also performed using active carbon/ash of paper and egg white/the tannic acid-calcium chloride. Results Satisfactory result was achieved in the three agglutinate reactions. Conclusion This method, which can be used as the simulating method of the three indirect agglutinations, is economy and efficient for experiments of immunology teaching.
Keywords:tex gatherscarbon  Erythrocyte agglutination  Catbon particle agglutination  Simulation
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