| 高效液相色谱法测定银黄注射液中绿原酸、黄芩苷和黄芩素含量 |
| |
| 引用本文： | 庞兴寿,周颖,黎晓萍.高效液相色谱法测定银黄注射液中绿原酸、黄芩苷和黄芩素含量[J].中国药业,2009,18(14):35-36. |
| |
| 作者姓名： | 庞兴寿 周颖 黎晓萍 |
| |
| 作者单位： | 广西壮族自治区玉林食品药品检验所,广西玉林,537000 |
| |
| 摘 要： | 目的建立银黄注射液中绿原酸、黄芩苷和黄芩素的含量测定方法。方法色谱柱为Agilent Zorbax SB—C18柱（250mm×4．6mm，5μm），流动相为乙腈-0．1％磷酸溶液（梯度洗脱），检测波长为318nm，流速为1．00mL／min，柱温为25℃。结果绿原酸、黄芩苷和黄芩素进样量分别在0．203。3．248μg，2．166～34．656μg，8．008×10^-3～160．16×10^-3μg范围内与峰面积线性关系良好；平均回收率（n=6）分别为96．98％，97．74％，96．64％。结论HPLC法简便、准确、重现性好，可用于银黄注射液的质量控制。
|
| 关 键 词： | 银黄注射液 梯度洗脱 绿原酸 黄芩苷 黄芩素 高效液相色谱法 含量 |
Determination of Chlorogenic Acid, Baicalin and Scutellarin in Yinhuang Injection by HPLC |
| |
| Pang Xingshou,Zhou Ying,Li Xiaoping.Determination of Chlorogenic Acid, Baicalin and Scutellarin in Yinhuang Injection by HPLC[J].China Pharmaceuticals,2009,18(14):35-36. |
| |
| Authors: | Pang Xingshou Zhou Ying Li Xiaoping |
| |
| Institution: | (Yulin Institute for Food and Drug Control, Yulin, Guangxi, China 537000) |
| |
| Abstract: | Objective To study and establish a HPLC method for the content determination of chlorogenic acid, baicalin and scutellarin in Yinhuang Injection. Methods Agilent Zorbax SB-C18 column（250 mm ×4. 6 mm, 5 μm） was used at 25 ℃. The mobile phase consisted of acetonitile-0. 1% phosphoric acid with gradient elntion, the detection wavelength was at 318 nm, the flow rates was 1.00 mL/min. Results The calibration curve of chlorogenie acid, baicalin and scutellarin were linear over the range of 0.203-3.248 μg, 2. 166-34.656 μg, 8. 008 × 10^-3 - 160. 16 × 10^-3 μg respectively. The average recovery rates were 96.98% , 97.74% , 96. 64% respectively. Conclusion The method is simple, effective with good reproducibility, which can be used for the quality control of Yinhuang Injection. |
| |
| Keywords: | Yinhuang Injection gradient elution chlorogenic acid baicalin scutellarin HPLC content |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
