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| 参附注射液预处理对大鼠脑缺血-再灌注损伤后HSP70和HSP90表达的影响 | |
| 引用本文： | 李扬,杨博,陈绍洋,汪晨,路志宏,马福成,熊利泽.参附注射液预处理对大鼠脑缺血-再灌注损伤后HSP70和HSP90表达的影响[J].临床麻醉学杂志,2010,26(1). |
| 作者姓名： | 李扬 杨博 陈绍洋 汪晨 路志宏 马福成 熊利泽 |
| 作者单位： | 1. 第四军医大学两京医院麻醉科,西安市,710032 2. 第四军医大学唐都医院门诊部膊 3. 第四军医大学两京医院病理科,西安市,710032 |
| 基金项目： | 国家自然科学基金资助项目(30571793) |
| 摘 要： | 目的探讨参附注射液(SF)单次预处理对大鼠脑缺血-再灌注损伤的保护作用。方法30只雄性SD大鼠随机均分为单纯缺血-再灌注组(MCAO组)、SF预处理组(SF组,缺血前30 min经腹腔注射SF 10 ml/kg)和假手术组(Sham组)。MCAO组和SF组采用颈内动脉尼龙线线栓法致右侧大脑中动脉栓塞120 min。观察再灌注后24 h时脑组织病理学改变,检测热休克蛋白(HSP)70和HSP90表达。结果再灌注24 h后,SF组脑组织病理学损害轻于MCAO组。Sham组未见HSP70及HSP90表达。MCAO组可见到较多的HSP70免疫阳性细胞,明显高于Sham组(P<0.01);SF组HSP70免疫阳性细胞分布较MCAO组广泛,数量明显增多,胞浆及胞核都可见HSP70免疫阳性细胞(1.341±0.464)vs.(0.856±0.574)](P<0.05),而SF组HSP90的表达与MCAO组相仿(0.006±0.013)vs.(0.005±0.007)]。结论SF预处理可通过增加HSP70表达而对大鼠脑缺血-再灌注损伤起保护作用。 |
| 关 键 词： | 参附注射液 缺血-再灌注损伤 热休克蛋白 |
Effects of shenfu injection pretreatment on the expression of HSP70 and HSP90 after cerebral ischemia-reperfusion injury in rats |
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| LI Yang,YANG Bo,CHEN Shao-Yang,et al..Effects of shenfu injection pretreatment on the expression of HSP70 and HSP90 after cerebral ischemia-reperfusion injury in rats[J].The Journal of Clinical Anesthesiology,2010,26(1). | |
| Authors: | LI Yang YANG Bo CHEN Shao-Yang |
| Institution: | LI Yang,YANG Bo,CHEN Shao-Yang,et al.Department of Anesthesiology,Xijing Hospital,The Fourth Military Medical University,Xi'an 710032,China |
| Abstract: | Objective To investigate the effects of shenfu injection(SF) pretreatment on the expressions of heat shock protein(HSP)70 and HSP90 after cerebral ischemia-reperfusion(I-R) injury in rats.Methods Thirty male SD rats were randomized into three groups of ischemia by the middle cerebral artery occlusion for 120 min(MCAO),SF 10 ml/kg pretreatment(SF,30 min before ischemia) and sham-operation(sham) with 10 rats each.The pathological changes and the expressions of HSP70 and HSP90 were detected at 24 h after reper... |
| Keywords: | Shenfu injection Ischemia-reperfusion injury Heat shock protein |
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