| 线栓法制备大鼠视网膜缺血再灌注模型的初步探讨 |
| |
| 引用本文： | 尹晓涛,孟瑞华,王景. 线栓法制备大鼠视网膜缺血再灌注模型的初步探讨[J]. 青岛大学医学院学报, 2008, 44(4) |
| |
| 作者姓名： | 尹晓涛 孟瑞华 王景 |
| |
| 作者单位： | 青岛大学医学院附属医院眼科,山东,青岛,266003;青岛大学医学院附属医院眼科,山东,青岛,266003;青岛大学医学院附属医院眼科,山东,青岛,266003 |
| |
| 摘 要： | 目的 建立一种理想的视网膜缺血再灌注动物模型.方法 成年Wistar大鼠20只,除2只4眼作为对照组外,余18只36眼作为实验组,分为缺血1 h组及缺血2 h组,每组9只.实验中用预先制备的线栓插入实验组大鼠颈内动脉,造成视网膜缺血,分别于缺血1、2 h后及再灌注6 h后观察眼底,术后摘除缺血1、2 h及再灌注6 h的眼球进行组织学观察.结果 实验组大鼠术后均发生视网膜缺血,视网膜苍白、水肿,以神经纤维层和内网状层最明显,可见白细胞浸润.再灌注6 h后视网膜水肿减退,神经纤维层水肿减轻,造模成功.结论 此方法比较接近视网膜缺血临床病理过程,模型稳定、可靠.
|
| 关 键 词： | 模型 动物 视网膜 缺血 再灌注 大鼠 |
ESTABLISHMENT OF RETINAL ISCHEMIA-REPERFUSION MODEL WITH THREAD OCCLUSION IN RAT:A PRELIMINARY STUDY |
| |
| YIN XIAO-TAO,MENG RUI-HUA,WANG JING. ESTABLISHMENT OF RETINAL ISCHEMIA-REPERFUSION MODEL WITH THREAD OCCLUSION IN RAT:A PRELIMINARY STUDY[J]. Acta Academiae Medicinae Qingdao Universitatis, 2008, 44(4) |
| |
| Authors: | YIN XIAO-TAO MENG RUI-HUA WANG JING |
| |
| Affiliation: | YIN XIAO-TAO,MENG RUI-HUA,WANG JING (Department of Ophthalmology,The Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China) |
| |
| Abstract: | Objective To establish an ideal retinal ischemia-reperfusion animal model. Methods A segment of prepared thread was introduced into the carotid artery of 18 adult Wistar rats and resulted in retinal ischemia of 36 eyes,which was followed by 6 h reperfusion.Fundus changes and morphological changes of eyes were observed after ischemia and reperfusion.According to the period of ischemia,the 36 eyes were divided into two groups: group 1,having 1 h ischemia,group 2,having 2 h ischemia.Four eyes of two rats serve... |
| |
| Keywords: | Models animal Retinal Ischemia Reperfusion Rats |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
