Interleukin-1 receptor antagonist in newborn babies and pregnant women |
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Authors: | V. Pillay N. Savage H. Laburn |
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Affiliation: | (1) The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, S-106 91 Stockholm, Sweden;(2) Institut für Zoologie, Universität Regensburg, D-93040 Regensburg, Germany |
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Abstract: | We have used patch-clamp techniques to study the effect of the sulfhydryl group oxidizing agents mercury and thimerosal on calcium-activated nonselective cation channels from brown adipose tissue. 100 nmol/l mercury and 50 mol/l thimerosal induced a complete block. Blockade could be reversed by reduction of the mercaptide by dithiotreitol (DTT). Mercury was found to be the most potent blocker (IC50-value 21×10–9 mol/l), whereas thimerosal (IC50-value 1.5×10–6 mol/l) was as effective as 3 ,5-dichlorodiphenylamine-2-carboxylic acid (DCDPC). The DCDPC effect, however, could not be reversed by DTT, indicating different blocking mechanisms. It is concluded that SH-groups are involved in gating of the calcium-activated nonselective channel. |
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Keywords: | Brown fat DCDPC Mercaptide Mercury Nonselective cation channel Sulfhydryl reagent Thimerosal |
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