S—100蛋白免疫酶技术对脂肪肉瘤的诊断和鉴别诊断的意义

本文报告用常规H·E染色、组织化学和免疫组织化学“三结合”的方法对47例脂肪肉瘤和有空泡性瘤细胞软组织肿瘤及瘤样病变进行研究。结果:本组有3例肿瘤分别修正为胚胎型横纹肌肉瘸、圆形细胞型脂肪肉瘤和恶性纤维组织细胞瘤。作者认为应用此“三结合”法对有空泡性瘤细胞软组织肿瘤及瘤样病变的诊断、鉴别诊断和确定肿瘤组织学类型是有所裨益,特别是在缺乏电镜设备的情况下。此外,S-100蛋白反应的强度与脂肪肉瘤分化的程度有关,提示有可能对脂肪肉瘤的恶性程度进行判测。

The Significance of S-100 Protein Immunoenzymologic Technique in Diagnosis and Differential Diagnosis of Liposarcoma

Forty seven cases of liposarcoma and soft tissue tumors and tumor lesions with vacuolated tumor cells are studied by means of the "Three in one"method which consists of H.E stain, histochemistry and immunochemistry. The results are:14 per 16 liposarcomas are S-100 protein positive;7 well differentiated liposarcomas are weakly positive or negative, 9 poorly differentiated liposarcomas show S-100 protein positive or strongly positive. Except one of 10 cases all the malignant fibrous histocytomas are S-100 protein negative. In the 47 cases 3 tumors are revised as embryonal rhabdomyosarcoma, round cell liposarcoma and malignant fibrous histocytoma respectively.It is believed that the "Three in one" method is useful in the diagnosis of soft tissue tumors and tumors and tumor lesions with vacuolated tumor cells, and in the differential diagnosis and confirming the histologic types,especially in the condition of lack of electron microscope device. The reactive intensity of S-100 protein is related to the degree of liposarcoma differentiation. It may be used to predict the degree of the malignancy of liposarcoma.