| 噬菌体生物扩增法检测肺结核患者痰标本的临床应用价值 |
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| 引用本文： | 辛茶香,诸纪芬,方育霞.噬菌体生物扩增法检测肺结核患者痰标本的临床应用价值[J].现代预防医学,2012,39(8):1990-1991. |
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| 作者姓名： | 辛茶香 诸纪芬 方育霞 |
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| 作者单位： | 江西省胸科医院检验科,江西南昌,330006 |
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| 基金项目： | 江西省卫生厅科技计划资助课题(20073163) |
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| 摘 要： | 目的探讨噬菌体生物扩增法对肺结核临床诊断的应用价值。方法对298例肺结核患者的痰标本,20例非结核性其他肺部疾病患者痰标本进行噬菌体生物扩增法检测,同份标本同时进行涂片抗酸染色、分枝杆菌罗氏培养。结果 298例痰标本有167例噬菌体生物扩增法检测结果阳性,阳性率56.0%;有78例涂片抗酸染色阳性,阳性率26.2%;有129例罗氏培养阳性,阳性率43.3%。结论噬菌体生物扩增法检测肺结核痰标本中结核分枝杆菌只需2d时间,操作简便、灵敏度高、特异性强,可作为诊断肺结核的一种检测方法。
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| 关 键 词： | 分枝杆菌 结核 分枝杆菌噬菌体 |
Application Value for the detection mycobacteria tuberlosis of sputum by Phage amplified biologically assay |
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| XIN Cha-xiang
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ZHU Ji-fen
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FANG Yu-xia.Application Value for the detection mycobacteria tuberlosis of sputum by Phage amplified biologically assay[J].Modern Preventive Medicine,2012,39(8):1990-1991. |
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| Authors: | XIN Cha-xiang ZHU Ji-fen FANG Yu-xia |
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| Institution: | .Jiangxi Chest Hospital,Nanchang,Jiangxi 330006,China |
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| Abstract: | OBJECTIVE To investigate the application value of phage amplified biologically(PhaB)assay in sputum of detection for pulmonary tuberculosis.METHODS The sputum of 298 patients with pulmonary tuberculosis and 20 patients with non-pulmonary tuberculosis,were detected by PhaB assay.RESULTS The positive rate of PhaB assay in 298 patients with pulmonary tuberculosis was 56.0%(167/298),the positive rate of culture was 43.3%(129/298),and the positive rate of smear was 26.2%(79/298).20 sputum with non-pulmonary tuberculosis was negative.CONCLUSION The experimental procedure used PhaB assay costs only 2 days,and it is simple,highly sensitive and specificity method for the diagnosis of pulmonary tuberculosis. |
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| Keywords: | Mycobacterium tuberculosis Mycobacteriophage |
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