吸毒人群外周血CD4~+CD25~+调节性T细胞和免疫功能变化及意义

目的通过测定不同吸毒方式的吸毒人群外周血中CD4+CD25+调节性T细胞(regulatoryTcell,Treg)的变化,从免疫调节方面探讨吸毒对人体免疫功能的影响以及机体的调节功能改变。方法清晨空腹抽取静脉血,采用流式细胞仪同期测定吸毒组和正常对照组人群CD4+CD25+Treg细胞和CD4+CD25+Foxp3Treg细胞及CD8+T淋巴细胞、CD4+T淋巴细胞的表达。结果口吸组和静脉注射组调节性T细胞与正常对照组比较,差异有统计学意义(LSD-t=2.34,4.35,P﹤0.05),静脉注射组和口吸组CD4+CD25+、CD4+CD25+Foxp3Treg细胞相比差异有统计学意义(LSD-t=3.37,4.07,P﹤0.05);不同吸毒途径淋巴细胞CD4+、CD8+表达不同,与对照组比较口吸组、静脉注射组CD4+和CD8+T淋巴细胞差异有统计学意义(LSD-t=5.18,4.94,2.17,2.23,P﹤0.05)。结论吸毒人群CD4+CD25+调节性T细胞功能代偿升高和不同吸毒方式导致机体免疫功能受损程度也不同。

Effect and changes of CD4~+ and CD25~+ regulatory T cells and immune function in the drug abuse population:Implications on changes caused by drug abuse

OBJECTIVE To investigate the effect of drug abuse on human immune function by examining the blood CD4+ CD25+ regulatory T cell expression,so as to explore the effect of drug abuse in human immunization and regulating function change. METHODS Blood samples were collected in the early morning before breakfast. Flow cytometry was employed to examine the expression of CD4+CD25+ T regulatory cells,CD4+CD25+Foxp3Treg cells,CD8+ T lymphocytes and CD4+ T lymphocytes in both drug abuse and normal population groups. RESULTS There were significant differences in regulatory T cells between oral drug abuse group and control group as well as between the intravenous injection drug abuse group and control group(LSD-t=2.34,4.35,P﹤0.05). Significant differences in CD4+CD25+ and CD4+CD25+Foxp3Treg cells were also found between intravenous injection and oral taken groups(LSD-t=3.37,4.07,P﹤0.05);There were also significant differences in the expression of CD4+ and CD8+ in the lymphocytes among four groups.(LSD-t=5.18,4.94,2.17,2.23,P﹤0.05). CONCLUSION There was a compensative increase in CD4+CD25+ regulatory T cells in drug abuse population. The extents of immune function injury were different among drug abuse people with different administration routes.