碱性成纤维细胞生长因子对慢性酒精中毒大鼠自由基与脂质过氧化作用的影响

目的观察碱性成纤维细胞生长因子(bFGF)对慢性酒精中毒大鼠脑和肝组织超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和羟自由基含量的影响,探讨bFGF对慢性酒精中毒所致的脑损伤、肝损伤的保护作用。方法选择成年Wistar雄性大鼠,采用白酒灌胃建立慢性酒精中毒模型,慢性酒精中毒模型建立成功的大鼠随机抽签法分为酒精中毒组、生理盐水(NS)对照组和bFGF治疗组,每组10只。另10只不灌白酒作为正常对照组。bFGF治疗组大鼠按12μg/kg剂量肌肉注射bFGF,共14d。各组大鼠到d14后取出各组大鼠脑、肝组织制成匀浆,测定脑、肝组织SOD活力、MDA含量和抑制羟自由基能力。结果与正常对照组相比,慢性酒精中毒后大鼠脑和肝组织SOD活力显著降低,MDA含量和羟自由基含量均明显升高(P﹤0.05);经bFGF治疗后脑和肝组织中SOD活力显著升高,MDA含量和羟自由基含量均显著降低。结论 bFGF起到降低自由基,提高机体的抗氧化能力,提示bFGF对慢性酒精中毒所致的脑损伤和和肝损伤具有保护作用。

Effects of bFGF on free radical and lipid peroxidation in cerebral cortex and liver tissue of rat model of alcoholism

OBJECTIVE To study the effects of basic fibroblast growth factor(bFGF)on the superoxide dismutase(SOD),malondialdehyde(MDA)and hydroxyl radical(OH.)in cerebral cortex and liver tissue of rat model of alcoholism.METHODS The rat model of alcoholism was established by perfusing stomach with alcohol.Wistar rats were randomly divided into of model of control group,model of alcoholism group,normal saline(NS)and bFGF treatment group.The SOD,MDA and(OH.)in cerebral cortex and liver tissue were detected.RESULTS MDA and(OH.)in cerebral cortex and liver tissue of rat model of alcoholism were significantly increased than those in control group,but the activities of SOD was significantly decreased than those in control group.After bFGF intervention,MDA and(OH.)in cerebral cortex and liver tissue of the bFGF group were significantly decreased compared with the NS group and model of alcoholism group respectively.But the activities of SOD were significantly increased.CONCLUSION bFGF possesses the reliable function of eliminating free radicals in cerebral cortex and liver tissue in alcohol induced alcoholism model rats.bFGF can protect alcoholic brain damage and liver damage in rats.