内蒙古包头市区肥胖儿童ApoE基因多态性及血脂水平

目的研究单纯性肥胖儿童ApoE基因多态性及血脂水平。方法检测来自本地区两所中学12～14岁60例肥胖儿童(60例对照)血脂,采用PCR限制性内切酶片段长度多态性(PCR-RFLP)技术分析ApoE基因多态性。结果本实验共检出4种ApoE基因型,分别是E3/3、E3/2、E3/4、E2/4,未检出E2/2、E4/4型。肥胖组与对照组等位基因ε2、ε3、ε4频率分布不均,ε3发生频率最高。与健康组儿童比较,肥胖组儿童ε4等位基因发生频率增高,差异有统计学意义(P﹤0.05)。肥胖组TC、TG、LDL-C水平由低到高依次为E2/X﹤E3/3﹤E4/X,HDL-C水平E2/X﹥E3/3,差异均有统计学意义(P﹤0.05)。对照组TC水平差异同肥胖组,LDL-C水平E2/X﹤E3/3,TG、HDL-C水平差异无统计学意义。结论 ApoE基因多态性影响肥胖儿童血脂水平。

Apolipoprotein E polymorphism and plasma lipid levels in obesity children in Baotou

OBJECTIVE To investigate ApoE polymorphism and plasma lipid levels in obesity children. METHODS We examined the ApoE genotypes and plasma lipids of 60 obese children(60 control children)from two middle schools aging from 12 to14 years old. ApoE genotyping was performed by polymerase chain resection-restriction fragment length polymorphism(PCR-RFLP). RESULTS In our research,4 genotypes of ApoE gene(E3/3,E3/2,E3/4 and E2/4)were detected. The allele frequency distribution was similar between the two groups and ε3 allele frequency distribution was the highest. Compared with control subjects,ε4 allele frequency distribution was higher(P﹤0.05). In obese group,TC,TG and LDL-C levels were E2/X﹤E3/3﹤E4/X(P﹤0.05),HDL-C level was E2/X﹥E3/3(P﹤0.05). In controls,TC level was the same in obesity group,and LDL-C level was E2/X﹤E3/3(P﹤0.05). CONCLUSION The results demonstrated that ApoE polymorphism did influence plasma lipid levels of obese children in our region.