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IL-23、IL-23 mRNA在皮肤移植受鼠外周血及移植物中的表达
引用本文:杜国盛,石炳毅,郑德华,宋继勇,朱志东,崔洪涛.IL-23、IL-23 mRNA在皮肤移植受鼠外周血及移植物中的表达[J].中华器官移植杂志,2010,31(11).
作者姓名:杜国盛  石炳毅  郑德华  宋继勇  朱志东  崔洪涛
作者单位:解放军第三○九医院器官移植中心,北京,100091
基金项目:全军"十一五"计划科研基金科技攻关课题 
摘    要:目的 探讨皮肤移植小鼠外周血及移植物中白细胞介素23(IL-23)和IL-23 mRNA的表达情况.方法 制作小鼠皮肤移植的动物模型:同系组供、受者均为Balb/c小鼠;同种组以C57BL/6小鼠为供者,Balb/c小鼠为受者;脾细胞组以C57BL/6小鼠为供者,Balb/c小鼠为受者,受鼠移植前1 d经尾静脉输注供者脾细胞1×107个/只.移植术后1、3、5和7 d留取受者血液和皮肤移植物样本,检测血清IL-23的浓度以及移植皮肤中IL-23 mRNA的表达,并于光镜下持续观察移植皮肤的病理学变化.结果 移植后第3和5天,同系组受鼠外周血IL-23浓度分别为(263.49±18.72)和(286.55±18.95)pg/ml,同种组分别为(295.66±16.36)和(332.58±17.37)pg/ml,脾细胞组分别为(303.66±16.99)和(323.96±18.99)pg/ml.移植后第3和5天,同系组受鼠外周血IL-23浓度低于同种组和脾细胞组(P<0.01);同种组和脾细胞组间的差异无统计学意义(P>0.05).皮肤移植后第3、5和7天,同系组受鼠移植皮肤IL-23 mRNA的相对表达量分别为0.57±0.10、0.63±0.10和0.66±0.11,同种组分别为0.78±0.09、0.81±0.09和0.69±0.14,脾细胞组分别为0.62±0.10、0.68±0.12和0.55±0.09.移植后第3和5天,同系组和脾细胞组受鼠移植皮肤IL-23 mRNA的相对表达量低于同种组(P<0.01).移植后第7天,同种组与脾细胞组受鼠移植皮肤IL-23 mRNA的相对表达量的差异有统计学意义(P<0.01).结论 皮肤移植后早期发生急性排斥反应时,受鼠高表达IL-23和IL-23 mRNA.IL-23可作为预测排斥反应发生的观察指标.

关 键 词:急性排斥反应  白细胞介素23  脾细胞

Expression of IL-23 and IL-23 mRNA in allograft and peripheral blood of mice subject to skin transplantation
DU Guo-sheng,SHI Bing-yi,ZHENG De-hua,SONG Ji-yong,ZHU Zhi-dong,CUI Hong-tao.Expression of IL-23 and IL-23 mRNA in allograft and peripheral blood of mice subject to skin transplantation[J].Chinese Journal of Organ Transplantation,2010,31(11).
Authors:DU Guo-sheng  SHI Bing-yi  ZHENG De-hua  SONG Ji-yong  ZHU Zhi-dong  CUI Hong-tao
Abstract:Objective To investigate the expression of IL-23 and IL-23 mRNA in allograft and peripheral blood of mice receiving skin transplantation under different immune states. Methods Mice skin allograft models were established and divided into 3 groups: synergeneic transplant group (BALB/c→BALB/c), allogeneic transplant group (C57BL/6→BALB/c), donor spleen cells infusion group (C57BL/6→BALB/c). Peripheral blood plasma concentration of IL-23 was measured by ELISA. RT-PCR was used to detect the expression of IL-23 mRNA in the skin allograft. Results There was no significant difference in the IL-23 and IL-23 mRNA expression among all three groups one day after skin transplantation (P>0. 05). On the day 3, 5, and 7 after skin transplantation, there was significant difference in the IL-23 and IL-23 mRNA expression levels between synergeneic transplant group, donor spleen cells infusion group and allogeneic transplant group (P < 0. 01 ), but there was no significant difference between synergeneic transplant group and donor spleen cells infusion group (P>0. 05). Conclusion The high expression levels of IL-23 and IL-23 mRNA were detected when early acute rejection took place in recipient mice. IL-23 could serve as a predictable and prognostic marker for the acute rejection. Infusion of donor spleen cells can significantly prolong the allograft survival.
Keywords:mRNA
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