八味护肝胶囊对酒精性肝纤维化大鼠模型的影响

目的探讨八味护肝胶囊对酒精性肝纤维化（ALF）大鼠模型的保护作用及其作用机制。方法将雄性Wistar大鼠随机分为4组：正常对照组、模型组、八味护肝胶囊组、益肝灵胶囊组。用烈性酒灌胃方法制备ALF大鼠模型。于16周末检测各组大鼠肝功能指标、肝纤维化指标及肝纤维化血清转化生长因子β1（TGF—β1）、金属蛋白酶组织抑制剂1（TIMP1）。结果与模型组比较，八味护肝胶囊组大鼠血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、总蛋白（TP）、透明质酸（HA）、层粘连蛋白（LN）、IV型胶原（CW）及TGF—β1、TIMP1明显降低（P〈0．01）。结论八味护肝胶囊对酒精性肝纤维化大鼠有较好的治疗作用；对TGF—β1、TIMP1的抑制作用可能是其治疗酒精性肝纤维化的机制之一。

STUDY ON THE EFFECTS OF BAWEIHUGAN CAPSULE ON ALCOHOLIC HEPATIC FIBROSIS IN RATS

Objective To investigate the protective effect and interactive mechanisms of Baweihugan capsule on alcoholic hepatic fibrosis （ALF） model in rats. Methods Wistar male rats were randomly divided into 4 groups ： normal group, model group, Baweihugan Capsule group and Yiganling capsule group. The model of alcoholic hepatic fibrosis was established by feeding alcohol liquor. At the end of 16 weeks treatment, the variety of Hepatic function ,the hepatic fibrosis indices, levels of serum TGF - β1 ,TIMP1 were measured. Results Compared with the model group,the level of serum ALT, AST, TP, HA, LN, CIV and TGF- β1 ,TIMP1 in Baweihugan capsule group were significantly decreased （ P 〈 0.01 ）. Conclusion Baweihugan capsule has beneficial effects in treatment of ALF in rats. As for TGF -β1, TIMP1 inhibitory effect may be one of the critical mechanism in ALF treatment.