血管内皮细胞生长因子与乙型肝炎病变的关系

目的研究血管内皮细胞生长因子（vascularendothelialgrowthfactor，VEGF）与乙型肝炎(乙肝)分级(G)和分期(S)的关系。方法250例乙肝患者肝活检标本用VEGF单克隆抗体进行免疫组织化学(S-P法)染色。结果VEGF在肝组织表达有胞浆型、膜窦型及窦内皮细胞型；VEGF主要由肝细胞分泌，其次在肝窦内皮细胞、贮脂细胞、成肌纤维细胞及单核细胞也示阳性。无明显病变的血管内皮细胞及肝细胞VEGF染色阴性。提示随分级增高VEGF表达增强，其间差异有极显著意义（χ

Expression of vascular endothelial growth factor in liver tissues of hepatitis B

Objective To study the relationship between vascular endothelial growth factor (VEGF) and grading (G) and staging (SS) of hepatitis B (HB). Methods Immunohistochemical staining was performed with VEGF monoclonal antibody in 250 biopsies of HB. Results The types of VEGF expression in liver tissues were of plasma type, sinusoidal membrane type and sinusoidal endothelial cell type. VEGF was mainly secreted by hepatocytes, and was also positively expressed in liver sinusoidal endothelial cells, fat-storing cells, myofibroblast and monomorphological nuclear cells. And it was negative in vascular endothelial cells and hepatocytes which had not significant damage, suggesting that the expression of VEGF was enhanced with the increase of G (x~2=90.43, P<0.01). VEGF was positively expressed on those whose scores of S were lower, while strongly positively expressed on those whose scores of S were higher. There was also a significant difference between them (x~2= 115.74, p<0.01). Conclusion The expressions of VEGF are related to G and S of HB.