Septal release of vasopressin in response to osmotic, hypovolemic and electrical stimulation in rats |
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| Authors: | J. Demotes-Mainard J. Chauveau F. Rodriguez J.D. Vincent D.A. Poulain |
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| Affiliation: | 1. Department of Neurosurgery, Ankara Numune Research and Education Hospital, Ankara, Turkey;2. Department of Neurosurgery, Ankara Research and Education Hospital, Ankara, Turkey;1. Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL;2. Department of Medical Social Sciences, Northwestern University Feinberg School of Medicine, Chicago, IL;1. 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Warsaw, Poland;2. Department of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Warsaw, Poland;1. Department of Psychiatry, Campus Charité Mitte, Charité – Universitätsmedizin Berlin Charitéplatz 1, 10117 Berlin, Germany;2. Department of Psychiatry, Universitätsklinikum Hamburg Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany;1. Centre de référence des Maladies Neuromusculaires, CHU Lille, 59000 Lille, France;2. Service de Neurologie et pathologie du mouvement, CHU Lille, 59000 Lille, France;3. CHU Lille, Hôpital Calmette, Service de Pneumologie et ImmunoAllergologie, Centre de Référence Constitutif des Maladies Pulmonaires Rares, Lille, France;4. Department of Pathology, Medical University of Warsaw, Warsaw, Poland;5. CHU Lille, Lung Function Department, University Lille, INSERM 1019, CNRS UMR 8204, Institut Pasteur de Lille, Center for Infection and Immunity of Lille, Lille, France;6. Service de Neurophysiologie clinique, CHU Lille, 59000 Lille, France;7. Department of Musculoskeletal Radiology, Lille University Hospital, rue du Professeur Emile Laine, 59037 Lille cedex, France |
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| Abstract: | The central release of vasopressin was studied in anesthetized rats using push-pull perfusions and radioimmunoassay of the hormone. A basal release was observed in the lateral septum and in the lateral ventricle, whereas no vasopressin was detected in the perfusates from the caudate nucleus. Under osmotic stimulation, vasopressin release increased up to 12 and 60 times basal levels following i.p. injections of 5 ml and 10 ml/kg b.wt. of 2 M NaCl, respectively. This increase was blocked by using a calcium-free perfusion medium containing 0.1 mM EGTA. In the lateral ventricle, osmotic stimulation (5 ml/kg of 2 M NaCl i.p.) had the same effect as in the septum. In the caudate nucleus, no release was observed. Hemorrhage also increased the septal release of vasopressin in 5 out of 6 animals tested. Electrical stimulation of the pituitary stalk and of the supraoptic nucleus was used to evoke the release of vasopressin into the bloodstream. Septal release slightly decreased during pituitary stalk stimulation, whereas it did increase during stimulation of the supraoptic region. Our results show that systemic stimuli for vasopressin release evoke both a peripheral and a septal release of the hormone. The dissociation of the effects of electrical stimulation of the pituitary stalk and of the supraoptic nucleus suggests, however, that the vasopressinergic neurones responsible for septal release are distinct from those which project to the neurohypophysis. |
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| Keywords: | vasopressin septum push-pull cannula hemmorrhage hypertonic stimulation magnocellular system |
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