The Cardioprotective Effect of Necrostatin Requires the Cyclophilin-D Component of the Mitochondrial Permeability Transition Pore |
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Authors: | S Y Lim S M Davidson M M Mocanu D M Yellon C C T Smith |
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Institution: | (1) The Hatter Cardiovascular Institute, University College London Hospital and Medical School, 67 Chenies Mews, London, WC1E 6HX, UK |
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Abstract: | Background Necrostatin (Nec-1) protects against ischemia–reperfusion (IR) injury in both brain and heart. We have previously reported
in this journal that necrostatin can delay opening of the mitochondrial permeability transition pore (MPTP) in isolated cardiomyocytes.
Aim The aim of the present study was to investigate in more detail the role played by the MPTP in necrostatin-mediated cardioprotection
employing mice lacking a key component of the MPTP, namely cyclophilin-D.
Method Anaesthetized wild type (WT) and cyclophilin-D knockout (Cyp-D−/−) mice underwent an open-chest procedure involving 30 min
of myocardial ischemia and 2 h of reperfusion, with subsequent infarct size assessed by triphenyltetrazolium staining. Nec-1,
given at reperfusion, significantly limited infarct size in WT mice (17.7 ± 3% vs. 54.3 ± 3%, P < 0.05) but not in Cyp-D−/− mice (28.3 ± 7% vs. 30.8 ± 6%, P > 0.05).
Conclusion The data obtained in Cyp-D−/− mice provide further evidence that Nec-1 protects against myocardial IR injury by modulating
MPTP opening at reperfusion. |
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Keywords: | necrostatin mitochondrial permeability transition pore cyclophilin-D |
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