Prostate cancer mediates osteoclastogenesis through two different pathways |
| |
Authors: | Inoue Hitoshi Nishimura Kazuo Oka Daizo Nakai Yasutomo Shiba Masahiro Tokizane Takashi Arai Yasuyuki Nakayama Masashi Shimizu Kiyonori Takaha Natsuki Nonomura Norio Okuyama Akihiko |
| |
Institution: | Department of Urology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. |
| |
Abstract: | The present study was undertaken to test the effects of prostate cancer cell lines (LNCaP, DU145, PC3, and MDA PCa 2b) on osteoclastogenesis. Crude conditioned medium (CM) from all four prostate cancer cell lines enhanced expression of the mRNA for receptor activator of NF-kappaB ligand (RANKL) in a mouse osteoblast cell line, MC3T3-E1; however, CM had no effect on expression of osteoprotegerin (OPG) mRNA. Coculture of MC3T3-E1 with prostate cancer cells yielded similar results. The number of mature osteoclasts induced by soluble RANKL increased significantly when osteoclast precursor cells were cultured with CM from LNCaP and DU145 cells. CM from LNCaP and DU145 cells also induced maturation from precursor in the absence of soluble RANKL, and this effect was not blocked by OPG. Addition of CM from DU145 cells increased expression of MMP-9 mRNA by osteoclast precursors. Our findings indicate that prostate cancer mediates osteoclastogenesis through induction of RANKL expression by osteoblasts and through direct actions on osteoclast precursors mediated by some factors other than RANKL. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|