2-Hydroxyethyl methacrylate as an inhibitor of matrix metalloproteinase-2 |
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| Authors: | Rodrigo V Carvalho Fabrício A Ogliari Ana P de Souza Adriana F Silva Cesar L Petzhold Sergio R P Line Evandro Piva Adriana Etges |
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| Institution: | Department of Operative Dentistry, School of Dentistry, Federal University of Pelotas, Rio Grande do Sul;;Department of Morphology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo;;Organic Chemistry Department, Chemistry Institute, Federal University of Rio Grande do Sul, Rio Grande do Sul;;Department of Oral Pathology, School of Dentistry, Federal University of Pelotas, Rio Grande do Sul, Brazil |
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| Abstract: | This study evaluated the effect of different concentrations of 2-hydroxyethyl methacrylate (HEMA) on the inhibition of matrix metalloproteinase-2 (MMP-2) in vitro . Mouse gingival explants were cultured overnight in Dulbecco's modified Eagle's minimal essential medium, following which the expression of secreted enzymes was analyzed by gelatin zymography and the effects of different amounts of HEMA on enzyme activity were investigated. The gelatinolytic proteinases present in the conditioned media were characterized as being matrix metalloproteinases (MMPs) by means of specific chemical inhibition. The MMPs present in the conditioned media were identified, using immunoprecipitation, as MMP-2. Three major bands were detected in the zymographic assays and were characterized, according to their respective molecular weights, into the following forms of MMP-2: zymogene (72 kDa), intermediate (66 kDa), and active (62 kDa). All forms of MMP-2 were inhibited by HEMA in a dose-dependent manner, implying that MMP-2 may be inhibited by HEMA in vivo . |
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| Keywords: | 2-hydroxyethyl methacrylate gelatinase A matrix metalloproteinase-2 matrix metalloproteinases polyacrylamide gel electrophoresis |
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