阿米洛利对家兔心肌缺血-再灌注损伤的保护作用

目的：观察H^＋-Na^＋泵受体拮抗剂对家兔心肌缺血一再灌注损伤中心肌细胞损伤及细胞凋亡的影响。方法：将实验动物随机分为手术模型组，阿米洛利组，氨苯蝶啶组，正常对照组。观察血清CK—MB和LDH含量和细胞凋亡及坏死情况。结果：阿米洛利组心肌细胞凋亡率明显下降，氨苯蝶啶组未见明显改变。结论：在心肌细胞缺血一再灌注过程中，细胞凋亡是首要的病理改变，阿米洛利可降低细胞凋亡率，其机理可能与细胞内Ca^2＋浓度的调节有关。

Protective effect of amiloride on myocardial ischemic -reperfusion injury in rabbit

Objective:To observe the influence of amiloride on cadiocyte apoptosis during ischemic-reperfusion injury.Methods: The rabbits were randomly divided into operation model group,amiloride treatment group,triamterene treatment group and normal control group.Serum MB isoenzyme of creatine kinase(CK-MB) and lactate dehydrogenase (LDH) were measured with automatic biochemistry analyzer,and the apoptosis rate of myocardial cell was detected by flow cytometry.Results: There was no significant difference in apoptosis rate of myocardial cell between amiloride treatment group and normal control group(P>0.05).Compared with operation model group and triamterene treatment group,the apoptosis rate was markedly reduced in amiloride treatment group(P<0.01).Conclusion: Apoptosis is the chief pathologic change during cardiac ischemic-reperfusion injury.Amiloride can reduce the apoptosis rate of myocardial cell in this process,which may be related with intracellular Ca2 concentration adjustment by amiloride.