银屑病角质形成细胞抗菌蛋白的表达水平比特应性皮炎高

最近，研究显示特应性皮炎患者皮损的抗菌肽表达水平比银屑病患者低。本文采用微阵列检测方法，分析慢性斑块性银屑病和慢性特应性皮炎患者纯化的皮损处表皮细胞的mRNA表达谱，研究是否为宿主防御蛋白的普遍现象，并探讨其特异性。选择一组基因用定量PCR和免疫组化证实微阵列的数据。研究发现，相对于特应性皮炎皮损，银屑病皮损角质形成细胞有许多抗菌蛋白过度表达。有意思的是，正常分化和激活／过度增殖表皮表型的标志物表达水平一致。就细胞增殖而言，银屑病和特应性皮炎患者的慢性皮损惊人相似。研究推断银屑病表皮宿主防御蛋白的表达水平比特应性皮炎表皮高，这种现象对这些蛋白是特异性的。这是由导致表皮抗菌反应的基因多态性所致还是由银屑病与特应性皮炎细胞浸润的差别引起，仍需进一步研究。

High expression levels of keratinocyte antimicrobial proteins in psoriasis compared with atopic dermatitis

Recently, it was shown that matitis patients expresses low lesional skin of atopic derlevels of some antimicrobial peptides, compared with psoriasis patients. Here we performed mieroarray analysis on mRNA from purified lesional epidermal cells of patients with chronic plaque psoriasis and chronic atopie dermatitis, to investigate whether this is a general phenomenon for host defense proteins, and how specific it is for this class of molecules. Mieroarray data were confirmed on a selected set of genes by quantitative PCR and at the protein level by immunohistoehemistry. We found overexpression of many antimierobial proteins in keratinoeytes from psoriatic skin compared with atopie dermatitis skin. Interestingly, we observed that markers of normal differentiation and the activated/hyper proliferative epidermal phenotype were expressed at equal levels. Chronic lesions of psoriasis and atopie dermatitis patients are remarkably similar with respect to cellular proliferation. We conclude that psoriatic epidermis expresses high levels of host defense proteins compared with atopie dermatitis epidermis, and this phenomenon appears to be specific for these proteins. It remains to be investigated whether this is caused by genetic polymorphisms in pathways leading to an epidermal antimierobial response, or by differences in the cellular infiltrate in psoriasis compared with atopie dermatitis.