基因芯片对哮喘小鼠趋化因子及其受体差异表达的筛选与分析

目的用SABioscience基因芯片检测小鼠哮喘模型中84个趋化因子及其受体基因,并初步筛选分析其与支气管哮喘的相关性。方法用OVA构建哮喘小鼠模型,于末次激发后取右肺组织进行HE染色,并提取左肺组织总RNA,用SABioscience芯片检测84个趋化因子及其受体基因的表达水平。结果成功构建哮喘小鼠模型,哮喘组与对照组相比,表达上调的趋化因子21个,趋化因子受体13个;表达下调的趋化因子4个。结论 SABioscience芯片可有效地筛选出哮喘小鼠差异表达的趋化因子及其受体,对进一步深入探讨哮喘的发病机制及预防具有重要意义。

Screening and analysis of chemokines and their receptors expression in murine asthma model using gene microarray

Objective To test the expression of 84 chemokines and chemokines receptors genes in murine asthma model using SABioscience gene microarray and preliminarily screen and analyze their relationship to asthma.Methods Murine asthma model was established through i.p.injection of OVA and aerosol challenge.Within24 hours after-last stimulating,the right lung tissue of mice was stained by HE staining.Total RNA was extracted from left lung tissue and the expression level of 84 chemokines and chemokine receptors were analyzed by SABioscience gene microarray.Results Murine asthma models was successfully established.21 chemokines and16 chemokine receptors are over two fold up-regulated and 4 chemokines are over two fold down-regulated in asthma group,compared with those in control group.Conclusion Differently expressed chemokines and chemokine receptors of asthma mice could be effectively filtered out by SABioscience microarray,which provided the foundation for further exploration of the pathogenesis mechanism and prevention of asthma.