人源间变性大细胞淋巴瘤BALB/c小鼠建模的免疫学机制

目的 建立人源间变性大细胞淋巴瘤（ALCL） BALB/c小鼠模型,探讨成瘤的免疫学机制.方法 BALB/c小鼠环磷酰胺（CTX）预处理,Karpas-299细胞右腋下注射;流式细胞术检测各组小鼠外周血淋巴细胞亚群比例.结果 ①成功建立人源间变性大细胞淋巴瘤BALB/c小鼠模型.②成瘤组与CTX组相比,CD3＋、CD8＋和CD19＋细胞减少（P＜0.05）;未成瘤组与CTX组相比,CD8＋、CD19＋细胞减少,CD20＋细胞增多（P＜0.05）;成瘤组CD3＋、CD8＋细胞较CTX组和未成瘤组均减少（P＜0.05）;未成瘤组CD20＋细胞较CTX组和成瘤组均增多（P＜0.05）.③注射CTX第3天,BALB/c小鼠CD3＋细胞增多,CD19＋细胞减少（P＜0.05）;第10天CD8＋、CD19＋、CD20＋细胞较注射CTX前减少（P＜0.05）;第17天和第24天,CD8＋、CD19＋、CD20＋细胞仍低于注射CTX前（P＜0.05）,但较第10天有逐渐回升趋势（P＞0.05）.结论 CTX所致机体免疫抑制在ALCL成瘤起始阶段起决定性作用,ALCL的生长可能与CD3＋、CD8＋细胞减少有关.

Immunosuppression and establishment of anaplastic large cell lymphoma in BALB/c mouse model

Objective To establish the BALB/c mouse model with human anaplastic large cell lymphoma （ALCL）, and to explore the immunological mechanisms of tumor formation. Methods With the Cytoxan （CTX） pretreatment, Karpas -299 cells were injected to armpits for building model. Peripheral blood was collected to detect T and B lymphocyte subgroup proportion by flow cytometry. Results 1 ）The BALB/c mouse models bearing human ALCL were successfully established. 2 ） In tumor group, CD3 ＋ , CD8 ＋ , CD19 ＋ cells were significantly reduced than that of the control group （ P 〈 0.05 ） ; In group without tumor, CD8 ＋ , CD19 ＋ cells were fewer, CD20＋ cells were increased than that of the control group （P 〈 0.05 ） ; In tumor group, compared with the control group and the group without tumor, CD3 ＋ , CD8 ＋ cells were significantly reduced （P 〈0.05 ） ; In the group without tumor, compared with the control group and the tumor group, CD20 ＋ cells was significantly increased （ P 〈 0.05）. 3） In CTX control group, on third day, compared with before the injection of CTX , the proportion of CD3 ＋ cells was increased, the proportion of CD19 ＋ cells was decreased; On the tenth day,the decline in percentage of CD8 ＋ , CD19 ＋ , CD20 ＋ cells was significant （P 〈 0.05 ） ; On the 17th and 24th days, CD8 ＋ , CD19 ＋ , CD20 ＋ cells ratio was still lower than before the injection of CTX（ P 〈 0.05 ）, but there was gradually up ward trend compared with the 10th day （P 〉 0. 05 ）. Conclusion Immunosuppression caused by CTX plays a determinant role in the beginning of tumor formation ; The rapid growth of the tumor may be associated with the decreased CD3 ＋ and CD8 ＋ cells.