Ubiquitous Presence in Mammalian Cells of Enzymatic Activity Specifically Cleaving 8-Hydroxyguanine-containing DNA |
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| Authors: | Fuyumi Yamamoto Hiroshi Kasai Tadayoshi Bessho Myung-Hee Chung Hideo Inoue Eiko Ohtsuka Tomokatsu Hori Susumu Nishimura |
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| Affiliation: | Biology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104, Japan;Department of Pharmacology, College of Medicine, Seoul National University, Seoul 110, Korea;Faculty of Pharmaceutical Sciences, Hokkaido University, N12-W6, Kita-ku, Sapporo 060, Japan;Department of Neurosurgery, School of Medicine, Tottori University, Nishi-machi, Yonago 683, Japan |
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| Abstract: | Here we report the finding of enzymatic activity that specifically cleaves DNA containing 8-hydroxyguanine (oh8Gua) residues in various mammalian cells. To detect this activity, we used a synthetic double-stranded DNA containing a single oh8Gua at a defined position as the substrate, and analyzed the products of enzymatic digestion by polyacrylamide gel electrophoresis. Two cleavage sites near the oh8Gua residue were detected with partially purified fractions from cow brain and rat liver, and also with preparations from all mammalian tissues examined. These results suggest that enzymatic activity for the removal of oh8Gua from DNA is widely distributed in mammalian cells. |
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| Keywords: | Key words 8-Hydroxyguanine Repair enzyme |
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