蒺藜皂苷对缺氧心肌细胞内蛋白激酶C的作用

目的：研究蒺藜皂苷(GSTT)对氰化钠（NaCN）诱导大鼠乳鼠心肌细胞缺氧时心肌细胞内蛋白激酶C含量的影响。方法:利用培养的Wistar大鼠乳鼠心肌细胞，采用NaCN建立心肌细胞内缺氧模型，100 mg·L^-1 GSTT组和30 mg·L^-1 GSTT组干预12 h后，应用激光共聚焦显微镜系统和流式细胞术定性定量研究心肌细胞内δPKC和εPKC的分布及含量。结果:GSTT能明显促进εPKC和δPKC从细胞浆向细胞膜的转位,100 mg·L^-1 GSTT组激光聚焦显微镜系统及流式细胞术测定的荧光定量心肌细胞内εPKC及δPKC（1 325.00±53.25、810.55±36.89；66.22±6.23、40.12±2.21）与模型组（792.00±32.36、492.40±30.15；32.70±2.78、29.28±4.82）比较，差异具有显著性（P<0.001，P<0.01；P<0.001，P<0.01），GSTT明显增加δPKC和εPKC表达，100 mg·L^-1 GSTT组δPKC和εPKC含量（1 325.00±53.25、810.55±36.89；66.22±6.23、40.12±2.21）高于30 mg·L^-1 GSTT组(998.00±23.21、710.00± 38.12;55.35±5.15、35.68±3.89)，两组比较，差异具有显著性（P<0.01，P<0.05；P<0.01，P<0.05）。结论：GSTT可能通过激活PKC以保护受损心肌细胞。

Effect of Gross Saponin Tribulus Terrestris on protein kinase C expression in neonate rat cardiocytes injured by hypoxia

Objective To study the protective mechanism of Gross Saponin Tribulus Terrestris(GSTT) on the neonatal rat ventricular cardiocytes injured by hypoxia and the effect on protein kinase C(PKC).Methods The hypoxia-ischemia model was performed by treating the cultured neonatal rat ventricular cardiocytes with NaCN.The effect of GSTT(100 and 30 mg·L~(-1)) on the contents of εPKC and δPKC were detected by flow cytometry and laser confocal microscopy system.Results GSTT up-regulated εPKC and δPKC expressions.The contents of εPKC and δPKC in GSTT 100 mg·L~(-1) group(1 325.00±53.25,810.55±36.89;66.22±6.23,40.12±2.21) were increased significantly than those in model group(792.00±32.36,492.40±30.15;32.70±2.78,29.28±4.82)(P<0.001,P<0.01;P<0.001,P<0.01),and were higher than those in GSTT 30 mg·L~(-1) group(998.00±23.21,710.00± 38.12;55.35±5.15,35.68±3.89)(P<0.01,P<0.05;P<0.01,P<0.05).(Conclusion GSTT can) protect cardiocytes through activating PKC signaling pathway.