不同显色方法对知母总皂苷含量测定的影响

目的:考察不同显色方法对紫外-可见分光光度法测定知母总皂苷含量的影响。方法:采用紫外-可见分光光度法测定知母总皂苷含量,分别用两种显色方法,显色剂方法1为香草醛-冰醋酸法,即香草醛-冰乙酸∶高氯酸∶冰乙酸(2∶8∶50),检测波长为545nm。显色方法2为浓硫酸法,即用浓硫酸∶甲醇(1∶4),检测波长为270nm。结果:采用方法1显色,知母皂苷A3在0.058~0.58mg范围内线性关系良好(r=0.9999),知母皂苷样品加样回收率分别为100.19%,RSD为2.81%;方法2显色,知母皂苷A3在0.0128~0.64mg范围内线性关系良好(r=0.9996),知母皂苷样品加样回收率分别为98.50%,RSD为5.04%。结论:综合精密度实验、重复性实验、准确度实验等指标,方法1明显优于方法2,且该方法简便、准确、灵敏、重现性好。

Effects of Two Different Chromogenic Agents on Determination of Content of Total Saponins in Anemarrhena

Objective:To investigate the effects of two different chromogenic agents on determination of the content of total saponins in Anemarrhena by UV spectrophotometry.Methods:Total Anemarrhenasaponin were determinated by two different chromogenic agents,namely method one,vanillin-glacial acetic acid perchlorie acid glacial acetic acid(2∶8∶50)at 545 nm;method two,sulfuric acid-methanol(1∶4) detected at 270 nm.Results:Using method one,the linear range of Anemarrhenasaponin A3 was 0.058～0.58 mg(r=0.9999).The recovery rate was 100.19%,RSD2.81%;using method two,the linear range of Anemarrhenasaponin A3 was 0.0128～0.64 mg(r=0.9996).The recovery rate was 98.50%,RSD4.96%.Conclusion:From these index,the first method was better than the second method.This method was simple,accurate and the reproducibility was good.