姜黄素和Dickkopf-1联用对口腔鳞状细胞癌细胞增殖和凋亡的影响研究

目的:探讨姜黄素和分泌蛋白DKK1(Dickkopf-1)联用对口腔鳞状细胞癌细胞增殖和凋亡的影响。方法:CCK8实验检测0、5、10、20、40、80μmol/L的姜黄素作用于人口腔鳞状细胞癌CAL27、Tca8113、SCC-4细胞24、48、72 h的细胞增殖情况,计算IC50值;实验分为对照组、姜黄素组、Dickkopf-1组、姜黄素+Dickkopf-1组,各组细胞处理48 h后,CCK8试验检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测Cleaved caspase3、β-catenin、CyclinD1蛋白表达。结果:不同浓度的姜黄素作用于CAL27、SCC-4、Tca8113细胞24、48、72 h后的细胞抑制率均显著高于0h时的细胞抑制率,且随着时间延长及浓度增加细胞抑制率增加(P<0.01),根据IC50值选择Tca8113细胞及30μmol/L的姜黄素作为后续研究。结论:姜黄素和Wnt/β-catenin信号通路抑制剂Dickkopf-1均能抑制口腔鳞状细胞癌细胞增殖和促进凋亡,两者联用的效果强于单独用姜黄素或Dickkopf-1。

Effects of Curcumin Combined with Dickkopf-1 on Proliferation and Apoptosis of Oral Squamous Cell Carcinoma Cells.

Objective: To investigate the effect of curcumin combined with Dickkopf-1 on proliferation and apoptosis of oral squamous cell carcinoma cells. Methods: Cell proliferation was detected after 0, 5, 10, 20, 40, 80μmol/L curcumin treated CAL27, Tca8113, SCC-4 cells for 24, 48 and 72h, and calculated the value of IC50. The cells were divided into control group, curcumin group, Dickkopf-1 group, and Dickkopf-1+curcumin group. After treatment for 48h, the cell proliferation was detected by CCK8 test, the apoptosis was detected by flow cytometry, and the expressions of Cleaved caspase3, β-catenin, and CyclinD1 protein were detected by Western blot. Results: The cell inhibition rates at different concentrations of curcumin treated CAL27, SCC-4 and Tca8113 cells for 24, 48 and 72h were significantly higher than the cell inhibition rate at 0h, and the cell inhibition rate increased with the prolongation of time and the increase of the concentration (P<0.01). According to the IC50 value, Tca8113 cells and 30 μmol/L curcumin were chosen as a follow-up study. Conclusion: Curcumin and Wnt/β-catenin pathway inhibitor Dickkopf-1 can inhibit the proliferation and promote apoptosis of oral squamous cell carcinoma cells. The combination effect of both is stronger than that of curcumin or Dickkopf-1 alone.