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| A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响 | |
| 引用本文： | 张晓艳,程俊,许华丽,毛亮,聂敏海.A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响[J].口腔医学研究,2017,33(4):440. |
| 作者姓名： | 张晓艳 程俊 许华丽 毛亮 聂敏海 |
| 作者单位： | 1. 西南医科大学附属口腔医院口腔内科 四川 泸州 646000; 2. 西南医科大学口颌面修复重建和再生实验室 四川 泸州 646000; 3. 西南医科大学电生理学教育部重点实验室 四川 泸州 646000 |
| 基金项目： | 四川省应用基础研究计划项目(编号:LY-62) |
| 摘 要： | 目的:探究A型钾离子(Potassium,K+)通道抑制后对舌鳞状细胞癌株(Tca8113)细胞的影响。方法:全细胞膜片钳技术记录和分析A型K+通道阻滞剂4-氨基吡啶(4-AP)对Tca8113细胞K+电流的影响;MTT检测A型K+通道对细胞增殖的作用。结果:在终浓度为2 mmol/L 4-AP作用下,A型K+电流的峰值从(267.04±13.84) pA降到(124.81 ±5.24) pA。在一定范围内,不同浓度4-AP能抑制Tca8113细胞在体外的增殖,且抑制程度随药物浓度和作用时间的增加而增强。结论:A型K+通道参与Tca8113细胞增殖的过程,且A型K+通道阻滞剂能抑制Tca8113细胞的增殖。 |
| 收稿时间： | 2016-09-28 |
Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells. |
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| ZHANG Xiao-yan,CHENG Jun,XU Hua-li,MAO Liang,NIE Min-hai..Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells.[J].Journal of Oral Science Research,2017,33(4):440. | |
| Authors: | ZHANG Xiao-yan CHENG Jun XU Hua-li MAO Liang NIE Min-hai |
| Institution: | 1. Dept. of Oral Medicine, The Affiliated Hospital of Stomatology, Southwest Medical University. Luzhou 646000, China; 2. Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Institute of Cardiovascular Medicine, Southwest Medical University. Luzhou 646000, China; 3. Key Laboratory of Medical Electrophysiology, Ministry of Education, Southwest Medical University. Luzhou 646000, China |
| Abstract: | Objective: To explore the effect of A-type potassium channels on the proliferation of Tca8113 cells. Methods: The whole cell patch clamp technique was used to record and analyze the inhibitory effect of 4-AP on A-type K+ current in Tca8113 cells, and MTT tests were used to test the inhibitory effect of 4-AP on cell proliferation in vitro. Results: Under the concentration of 2 mmol/L 4-AP, the peak of A type K+ current was decreased from (267.04±13.84)pA to (124.81 ±5.24)pA. The different concentrations of 4-AP could inhibit the proliferation of Tca8113 cells, and the magnitude of inhibitive effect was concentrations and reaction time dependent. Conclusion: The A-type of K+ channels play a role in the process of Tca8113 cell proliferation, and the blocker of A-type K+ channel can inhibit the proliferation of Tca8113 cells. |
| Keywords: | Tongue neoplasms Squamous cell 4-amino pyridine Potassium channels Voltage-gated Cell proliferation |
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