| PKCδ在丹酚酸B抗对乙酰氨基酚肝损伤中的作用研究 |
| |
| 引用本文： | 林木森,翟晓涵,范青,李桂茹,吕慧怡,张宁,胡艳,费淑香,李昀,路阳,姚继红. PKCδ在丹酚酸B抗对乙酰氨基酚肝损伤中的作用研究[J]. 中国医院药学杂志, 2016, 36(16): 1343-1346. DOI: 10.13286/j.cnki.chinhosppharmacyj.2016.16.02 |
| |
| 作者姓名： | 林木森 翟晓涵 范青 李桂茹 吕慧怡 张宁 胡艳 费淑香 李昀 路阳 姚继红 |
| |
| 作者单位： | 1. 大连医科大学附属第二医院药学部, 辽宁 大连 116023;2. 大连医科大学药理教研室, 辽宁 大连 116044;3. 大连医科大学附属第一医院药学部, 辽宁 大连 116022;4. 大连市妇产医院药剂科, 辽宁 大连 116021 |
| |
| 基金项目： | 国家自然科学基金（编号：81173641） |
| |
| 摘 要： | 目的： 探讨PKCδ在丹酚酸B（SalB）抗对乙酰氨基酚（APAP）肝损伤中的作用。方法： 通过MTT法、细胞内还原型GSH含量测定、细胞LDH溢出量测定检测SalB抗APAP肝损伤作用。Western Blot法检测SalB对Nrf2核移位的影响。应用PKCδ抑制剂或siRNA干扰技术，探索PKCδ在SalB抗APAP肝损伤中的作用，Western Blot法检测PKCδ表达及Nrf2激活。结果： SalB可明显减轻APAP造成的HepG2细胞损伤，同时激活PKCδ，促进Nrf2核移位。PKCδ选择性阻断剂Rottlerin可减弱以上保护作用。PKCδ敲除明显减弱SalB对Nrf2核移位的诱导作用。结论： SalB可减轻APAP所致肝细胞损伤，其机制与SalB通过PKCδ激活Nrf2通路有关。
|
| 关 键 词： | PKCδ 丹酚酸B 对乙酰氨基酚肝损伤 Nrf2 |
| 收稿时间： | 2016-01-04 |
PKCδ related signal pathway involves in protective effects of salvianolic acid B against acetaminophen induced hepatotoxicity |
| |
| LIN Mu-sen,ZHAI Xiao-han,FAN Qing,LI Gui-ru,LYU Hui-yi,ZHANG Ning,HU Yan,FEI Shu-xiang,LI Yun,LU Yang,YAO Ji-hong. PKCδ related signal pathway involves in protective effects of salvianolic acid B against acetaminophen induced hepatotoxicity[J]. Chinese Journal of Hospital Pharmacy, 2016, 36(16): 1343-1346. DOI: 10.13286/j.cnki.chinhosppharmacyj.2016.16.02 |
| |
| Authors: | LIN Mu-sen ZHAI Xiao-han FAN Qing LI Gui-ru LYU Hui-yi ZHANG Ning HU Yan FEI Shu-xiang LI Yun LU Yang YAO Ji-hong |
| |
| Affiliation: | 1. Department of Pharmacy, Second Affiliated Hospital of Dalian Medical University, Liaoning Dalian 116023, China;2. Department of Pharmacology, Dalian Medical University, Liaoning Dalian 116044, China;3. Department of Pharmacy, First Affiliated Hospital of Dalian Medical University, Liaoning Dalian 116022, China;4. Dalian Maternity Hospital, Liaoning Dalian 116021, China |
| |
| Abstract: | OBJECTIVE To explore potential roles of PKCδ in protective effects afforded by salvianolic acid B (SalB) against acetaminophen (APAP) induced acute liver injury.METHODS Protective effects of SalB against APAP-induced cell damage were characterized by measuring cell viability,GSH content and LDH level.Activation of Nrf2 by SalB was determined by Western-blot.To further explore roles of PKCδ involved,cells were treated with specific inhibitor or siRNA of PKCδ.Then expression of PKCδ and activation of Nrf2 were tested by Western-blot.RESULTS SalB ameliorated APAP-induced acute liver injury,which was correlated with Nrf2 translocation and PKCδ activation.Rottlerin,a specific inhibitor of PKCδ,markedly reduced protective effects afforded by SalB.In addition,PKCδ knock down significantly abolished activation of Nrf2 induced by SalB.CONCLUSION SalB protects against APAP-induced liver injury via activation of PKCδ/Nrf2 pathways. |
| |
| Keywords: | PKCδ salvianolic acid B acetaminophen induced liver injury Nrf2 |
|
| 点击此处可从《中国医院药学杂志》浏览原始摘要信息 |
|
点击此处可从《中国医院药学杂志》下载免费的PDF全文 |
|
