TRPAl在大鼠牙本质敏感牙髓组织中的表达研究

目的:研究机械刺激诱导大鼠牙本质敏感牙齿牙髓组织TRPAl的表达变化。方法:大鼠下切牙牙体预备后,每天用探针机械刺激暴露的牙本质,观察大鼠牙本质敏感行为学变化。7 d后,用SEM观察对牙本质表面形态;用RT-PCR 和Western Blot检测大鼠牙髓组织TRPAl的表达变化。结果:牙体预备后,大部分牙本质小管暴露开放,且无穿髓处;刺激组大鼠均出现了牙本质敏感反应行为学异常,其反应评分均显著高于对照组(P＜0.01),且强度增加,行为学反应更为剧烈。刺激组大鼠牙髓组织TRPA1 mRNA和蛋白的表达水平均显著升高,且随刺激强度增加,表达上调更为显著。结论:TRPA1可能是参与机械刺激相关性牙本质敏感发病的重要因子。

Expression of TRPAl in Rats Dental Pulp Induced by Dentin Hypersensitivity.

Objective: To study the expression of TRPAl in the dental pulp of dentin hypersensitivity teeth after mechanical stimuli. Methods: Cavities were prepared in the lower incisors of rats. After dentin exposure, dentin hypersensitivity test was performed by probe stimulation on dentin surface everyday. After 7 days, the dentin surface was observed by scanning electron microscopy (SEM). The dental tissue was harvest and underwent further RT-PCR and Western Blot analysis to investigate the expression of TRPAl in dental pulp. Results: After tooth preparation, most of dentinal tubules were open without pulp exposure. The rats of two stimulation groups appeared dentin hypersensitivity reaction behavior. The scores were significantly higher than that of the control group (P<0.01), and the intensity was increased with the intense of the behavioral reaction. The expression of TRPA1 mRNA and protein were significantly increased in the dental pulp of two stimulation groups, which was more significant with the increase of intensity of stimulation. Conclusion: TRPA1 may be an important pathogenesis factor of dentin hypersensitivity involved with mechanical stimuli.