肠外致病性大肠埃希菌毒力与耐药的相关性分析

Association of virulence and resistance of extraintestinal pathogenic E.coli

Objective :To investigate the association of extra-intestinal pathogenic E.coli (ExPEC) clinical isolates and resistance. Methods:A total of 187 ExPEC clinical isolates were investigated. Multiplex PCR and the minimum inhibitory concentrations (MICs) of antimicrobials were performed to detect the virulence factors and resistance among the 187 E. coli strains isolates. The comparisons of proportions between groups were carried out by using χ² test and Logistic regression. Results:The virulence factor genes kpsMT II, papC, PapEF, papG allele II (Internal), papA, cnf1, sfa/focDE, K5 and rfc had higher detection rates in non-urinary tract group than urinary tract group. The antimicrobial drugs CIP, LVX and TZP had higher resistance rates in urinary tract group than non-urinary tract group. Logistic regression analysis showed 9 kinds of virulence factor genes detected were independent protective factors for resistance of ExPEC, and one was independent risk factors for resistance of ExPEC. And another one had the two functions. Conclusion:Due to the different microenvironment and the selection pressure of antibiotics, the detection rate of virulence factor genes and the resistance rate of ExPEC are different, and the two are closely related.